The Experimental Study Of The Panaxnotoginseng Saponins Reversing K562/VCR Cell Multidrug Resistance In Vitro

Objective To search a sort of safe and effective reversal agent for multidrug resistantce from Chinese traditional medicine. And study the impact of the panaxnotoginseng saponins(PNS) to human leukemia clone K562 and multidrug resistance clone K562/VCR in vitro. Method to measure direct cell toxicant effect of PNS to K562(senstive strain) and K562/VCR(multidrug-resistant strain) and measure toxicant effect of dox to both strains for resistant index by MTT; To observe the reverse effect of PNS which has screened for nontoxicant concentration to K562/VCR by MTT; to measure concentration of dox in both sort of cell after applied PNS (200ug/ml). To measure expressional positve rate of the both cell after applied PNS (200ug/ml). Applied Veraparmil as a positive control to experiment aboveResult (1) there is no obvious cell toxicity of PNS to K562 and K562/VCR cell, when the concentration of PNS is 300ug/ml below. (2) Inhibitive concentration 50 of Dox to K562 and k562/VCR are 28.7ng/ml and 1141.8ng/ml respectively. Drug-Resistant index of K562/VCR is 39.7. (3) PNS can increased cell toxic effect of dox , after applied PNS of 50ug/mk 100ug/mk 200ug/ml concentration respectively to K562/VCR for 24 hours, inhibitive concentration 50 respectively decline to 392.8 ± 58.1ng/mK 282.6±53.8ng/ml, 203.6 ±19.75ng/ml(P<0.01): (4) PNS can increased K562/VCR's accumulated concentration of dox ,after applied PNS of 200ug/ml to K562/VCR for 3hours, find that fluorescenceintension increased from 108.36+11.73 tol45.76?.51 on average by flow cytometry(P<0.01). (5) measuring P-gp expression of mdr-1 of drug-resista-nt cell K562/VCR by flow cytometry after applied PNS and Ig-a(monoclony antibody of P-170) to K562/VCR for 3 hours ,indicate that average fluroescence intension of P-gp of expression decline from 138 to 65 .in contrast find no significant effect with verapamil. Conclusion (1) PNS of high concentration has certain tumour-resistant effect;.(2) PNS can increased K562/VCR's accumulated concentration of dox;(3) PNS can reduced K562/VCR's P-gp expression of mdr-1;(4) PNS can partly revese multidrug-resistance of K562/VCR in vitro;(5) PNS may be a kind of safe and efficient reversal agent for clinic.