| Objective:To investigate the effect of Valsartan on the balance between Th17and Treg in Chronic Viral Myocarditis (VMC)Methods:Balb/c mice(n=36)were infected with CVB3at days1,14,28to establish Chronic VMC models.The volumes of CVB3were0.20,0.25and0.30mL respectively.A normal control group(n=6) received an equal-volumes normal saline without CVB3at the same time.The mice survived in the CVMC group were randomly divided into the CVMC control group and Valsartan therapy group at day42th.From then on,mice of Valsartan treated group were administered Valsartan10mg/(kg.d) for28days and blood pressure was measured regularly. The severity of myocarditis was assessed by haematoxylin-eosin (HE) and Masson’s trichrome. The ratio of Th17and Treg cells was evaluated by Flow Cytometry.The RNA of IL-17A and foxp3were tested by Real-time PCR.Echocardiography was performed to evaluate the cardiac function of the mice.Data were reported as means±standard deviation (SD). For clinical scores, significance between each two groups was examined by using the Mann-Whitney U test. All other statistics were analyzed by using a one-way multiple-range ANOVA test for multiple comparisons. P≦0.05was considered significant.Results: There was no significant difference in the blood pressure level between three groups(p>0.05). Compared with the Valsartan group and normal control group, the heart weight to body weight radio, the percentage of CD4+thl7cell and cytokine of IL-17in the VCMC group,were significantly up-regulated while the pencentage of CD4+CD25+treg cell and cytokine of foxp3was down-regulated (p<0.01,p<0.01,p>0.05respectively). The AMA was down-regulated in treatment group compared with untreatment group.Conclusions:The results suggest that Valsartan can ameliorate viral myocarditis, may be due to their immunomodulatory reactions in the modification balance in generation of CD4+CD25+FoxP3+T cells to inhibit Th17cells induce autoimmune tissue injury. |
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