Effect Of Endogenous Hydrogen Sulfide On Viral Replication Of Myocardium In Coxsackievirus B₃ Induced Viral Myocarditis Of Mice

Objective: To observe the endogenous hydrogen sulfide(H₂S) on viral replication of myocardium in coxsackievirus B₃(CVB₃) induced viral myocarditis (VMC)of mice,and to explore the endogenous H₂S on pathogenesis of VMC .Methods:Male Balb/c mice were inoculated intraperitonelly(only one time)with 0.1ml of CVB₃ suspended liquid of 10-2 tissue culture infectious dose 50·L-1 to reconstrute an animal model of myocarditis. Seventy-five male Balb/c mice were randomly divided into five groups (n=15): normal control group,VMC group, D,L-prepargylycine[PAG,an irreversible inhibitor of cystathionine-γ-lyase(CSE)] low-dose,middle-dose and high-dose intervention groups, PAG was administrated by subcutaneous injection (sc).The mice were inoculated intraperitoneally with CVB₃ while sc PAG(the dosage of PAG in three intervention groups were 20.0,40.0,80.0mg·kg-1,respectivelly).each day one time for six times.All mice were sacrificed at seven days after inoculated intraperitoneally with CVB₃.The pathological changes of myocardial tissues were observed with light microscope.The content of H₂S was determined by spectrophotometry in myocardial tissue.The activity of CSE and content of cardiac troponinâ… (cTnâ… ) were determined by enzyme-linked immunosorbnent assay method in myocardial tissue and serum,respectively.The expression of CVB₃mRNA was detected by reverse transcription-ploymerase chaine reaction in myocardial tissue.Results: VMC group compared with normal control group:The activity of CSE and content of H₂S in myocardial tissues were significantly lower than those in normal control group(P﹤0.01),the pathological scores and expressive level of CVB₃mRNA in myocardial tissues,the content of cTnâ… in serum were significantly higher than those in normal control group(P﹤0.01),PAG three intervention groups compared with VMC group:The activity of CSE and content of H₂S in myocardial tissues were significantly lower than those in VMC group(P﹤0.01),the pathological scores and expressive level of CVB₃mRNA in myocardial tissues,the content of cTnâ… in serum were significantly higher than those in VMC group(P﹤0.01).The changes were obviously dose-dependent with PAG in PAG three intervention groups.Conclusion: The CVB₃ replication was inhibited by endogenous H₂S in myocardium.The CVB₃ replication related to reduced the activity of CSE in myocardium infected by CVB₃,thereby reduced the generation of endogenous H₂S in myocardium.