Preparation Of Silk Fibroin Peptide Applicated In Drug Controlled-Release And Study Of Its Effect

Drug delivery systems for controlled-release have many advantages, it can reduce thetimes of drug delivery, maintain the blood concentration, improve bioavailability andefficacy etc. The controlled-release systems can make great contributions to the clinicaltreatment, so, it has become one of the hot topics in the field of new drug preparations inrecent years. And the drug delivery support material plays an important role in thissystem, it directly affect the release effect. The researchers screened a large number ofnatural and synthetic materials, such as polyactic acid, cyclodextrin, hydroxyl ethylcellulose sustained-release materials. Silk fibroin as a natural protein,be founded can beused as drug controlled-released materials. Silk fibroin (SF) as a sustainable proteinresources has good biocompatibility, good biodegradability, good nutritional and healthyfunctions, non-immunogenic, non-toxic etc. This paper made a kind of blend material asdrug delivery support material, which was made by silk fibroin peptide(SFP), poly(vinylalcohol)(PVA), poly(ethylene glycol)(PEG) was made. And, its released effect was alsostudied.In this topic, a kind of SFP with low molecular weight, lessβ-sheet structure, morereactive side groups was received from large molecular weight through the enzymolysis ofthe neutral protease. Blend materials of SFP, PVA, PEG were prepared in water underdifferent polyethylene glycol(PEG) radios.The mechanical properties and scanningelectron microscopy results showed that the best performance is(SFP/PVA)/PEG=90:10(SFP:PVA=30:70). Infrared spectroscopy (IR), X-ray diffraction(XRD), differential scanning calorimetry (DSC) showed that the mechanical properties andthe compatibility was improved by PEG. Corroded mechanisms of the blend materials wasstudied under the human gastric environment, and found that the blend materials weredegraded through SF dissolved out first, then PEG losed slowly, and PVA losed finally. Thecell toxicity test was made by MTT. The result showed that the cytotoxicity of all thesamples was graded “0”or “1”by the criteria. There was no cytotoxicity with the compound,but also promoted the growth of L-929cells at the certain extent. The release experiment invitro was made through adding the drug of sulfate-hydroxychloroquine to the blend filmsevenly. And the result showed that the controlled-release action in the release medium(pH=6.8) was better than the acidic release medium. Its release action comformedfirst-order kinetics equation very well, which is a basic model of controlled-release system.And the action was also comformed Peppas,Higuchi release kinetic equation. So, we speculate that the blend materical have the potentiality as drug delivery support material indrug controlled-release system, especially in enteric-coated controlled-release preparation.