Preparation Of Silk Fibroin Biomedical Material And Controlled Drug Release Of Physiological Conditions

With the research on the structure and function of the silk fibroin goes deeply, the application of silk fibroin changes greatly, Its application no longer confined to textiles, but already extended to non-textile areas, such as pharmaceutical chemical, health care products and environmental detection. At the same time, the silk fibroin show the immeasurable potential for the development of tissue engineering, because of their impressive mechanical properties as well as the permeability and non-toxic to organisms.In this word, Silk fibroin (SF) films were prepared using a casting method and the change of secondary structure and crystal structure due to treatment with methanol-water and ethanol-water mixtures of different concentrations was investigated by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results were used to reveal the influence of structure of SF matrix on the release kinetics of drug compounds incorporated. It was found that the content of silk Ⅱ structure, constituted by β-sheet, first increased and then decreased with increasing alcohol concentration ranging from50%to90%, reaching a maximum around80%in methanol concentration and reaching a maximum around70～80%in ethanol concentration. The release kinetics of Rhodamine B, a model compound for small molecule drugs, was described by Peppas equation. The results indicated that Rhodamine B released from SF films via Fickian diffusion mechanism and the diffusional exponent n increased with increasing β-sheet content in the matrix. The experimental data had a good linear fit, suggesting that the silk Ⅱ crystal could be used as a natural regulator for drug release from SF material.Furthermore, the Silk fibroin/Glycerol blend Films were prepared using a casting method and the change of secondary structure and crystal structure was investigated by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results were used to reveal the influence of structure of SF matrix on the release kinetics of drug compounds incorporated. It was found that the content of silk Ⅱ structure, constituted by β-sheet, increased with increasing Glycerol concentration. Rhodamine B released from blend films via Fickian diffusion mechanism and the diffusional exponent n increased with increasing β-sheet content in the matrix. Glycerin could improve the drug release effect of silk fibroin as the carrier.