| ObjectiveTo investigate the polylactic acid polyglycolic acid copolymer/Ciliary neurotrophicfactor nerve conduit bridging repair of rat sciatic nerve defects induced by nerveregeneration.MethodIn tihs syudy,three-dimensional gel with CNTF (ciliary neurotrophic growth factor) ofpolylactic acid polyglycolic acid copolymer nerve conduit (Jinan City, Shandong Province,Dai Gang Biotechnology Co., Ltd. Preparation) bridging to repair defects of rat sciaticnerve is about10mm and polylactic acid polyglycolic acid copolymer, not added to thethree-dimensional gel of the CNTF nerve conduit to bridge repair and autologous nervegraft to repair a group of experimental animals (healthy wistar rats provided byexperimental Animal Center of Shandong University) formed the control group.Grossobservation after observation of each group of wound healing and the healing of plantarulcers, limb activities. After14weeks anatomy revealed the bridging part of the sciaticnerve, the gross morphology and the bridge part of the nerve regeneration was observedcatheter, and regeneration after nerve electrophysiological detection of bridging nerve withor without the action potential and the potential peak, take the renewable part nerve in1/3line pathological examination, observation of the regenerated nerve diameter and fibernumber, the analysis of nerve regeneration. Said take wet weight and muscle fibercross-sectional area measurement and evaluation of regenerated nerve after removal ofconnective tissue through the material of the three muscles of the ipsilateral leg. In thisstudy, male Wistar (SPF grade) to rats30, weight220-250g, with a random number table,the animals were divided into three groups (n=10). Each group with intraperitonealinjection of ketamine hydrochloride anesthesia (100mg/kg) respectively under themicroscope revealed that the left hind piriformis and reveal the sciatic nerve and piriformmargin of about5mm at the removal of a sciatic nerve and any of its natural retraction made about10mm nerve defect and handled accordingly, A group of rows with CNTFcopolymer material of polylactic acid polyglycolic acid nerve conduit bridging to repair thedefect. Group B simple line of poly lactic acid glycolic acid copolymer nerve conduits torepair the defect. Group C nerve autograft to repair the defect. After14weeks, grossobservation, nerve electrophysiological testing and the drawn line histological examinationto evaluate nerve regeneration.ResultsThe experimental group and the autograft group14weeks after surgery, limbclaudication and foot ulcers compared with poly nerve conduit group of the lactic acidpolyglycolic acid copolymer to reduce recovery effect. Nerve conduit have been dissolvedabsorption, polylactic acid polyglycolic acid copolymer/Ciliary neurotrophic factor nerveconduit bridging group14weeks after the regeneration of nerve longer than the defectsegment, the nerve diameter thick of the normal diameter of the nerve, nerveelectrophysiological testing found that the functional recovery of nerve conduction.Histological pathological sections showed that the experimental group and autograft grouphad more nerve fibers through the regeneration of nerve fibers in the wrong to the growthof the proportion of more polylactic acid poly-glycolic acid nerve conduit group of smallnerve fiber regeneration uniform size, maturity and good myelination, the triceps suraemuscle wet weight and myocyte cross-sectional area: group A and group C, P>0.05notstatistically significant; group A and group C were better than group B, P <0.05differencestatistically significant.ConclusionThis gel state with nerve growth factor (CNTF) nerve conduit in rat sciatic nervedefect induced regeneration, with the promotion of axon regeneration and reduce theproportion of regeneration of nerve wrong to growth, is expected to become autologousnerves alternatives. In this study provides an experimental basis for clinical applications.Further optimization of the nerve autograft substitute for clinical peripheral nerve defectshas important significance. |
PDF Full Text Request