The Experiment Study Of The Peripheral Nerve Regeneration

Objective Polylatic acid conduits are generally regarded as compound ncrvebirdging for repair of peripheral nerve defect. This article introduces the influence of peripheral nerve regeneration through polylactic acid conduits with different wall thick in the sciatic nerve in the rats. Methods Forty-five wister rats were randomly divided into three groups. Three kinds of polylactic acid conduits with 0.1, 0.3, 0.5mm wall thick bridged single lateral sciatic nerve defects for 10mm long. These conduits were injected into same concentration mixed filuds with schwann cell (SC) and Bletilla Striata gelatin (BSG), In order to improve sciatic nerve regeneration rate in three kinds of conduits. At 8, 12 weeks after the operation, we evaluated the nerve regeneration with histological method, neuroelectrophysiological examination and microdissective technique. Results The conduits with 0.3mm wall thick contain a large number of regenerated myelinated axons. They can enhance axonal regeneration and promote axonal myelination as well as improving recovery of the sciatic nerve funtion. The walls of the conduit become thin, but not soft deform, the conduits with O.lmm and 0.3mm wall thick contain less number of regenerated myelinated axons. After 12 weeks the walls of conduits with 0. 1mm thick are largely absorbed. On the other hard, the 0. 5mm thick walls become thicker than before. Conclusion The polylactic acid conduits in 0. 3mm wall thick which can enhance regeneration of myelinated axons are the best guide tube for perpheral nerve regeneration.