In Vitro And In Vivo Study Of The Chitosan Genes Nanoparticle In Liver Cancer

Purpose:Explore the effect and mechanism of chitosan gene nanoparticles on liver tumor growth and metastasis, preparation of a suitable gene carrier in the treatment of liver cancer, and provide valuable theoretical support for the treatment of liver cancerSubjects:SMMC-7721cells, nude mice liver tumorMaterials and methods:1) Preparation and characterization of chitosan gene nanoparticle:Preparation of maleic chitosan nanoparticles radical polymerization method through the interface; physical and chemical properties of the drug-loaded nanoparticles, including infrared spectroscopy, and surface morphology analysis, particle size analysis and stability analysis; study containedvitro release kinetics of drugs in the drug-loaded chitosan nanoparticles, the analysis of drug in vitro release kinetics of factors.2) In vitro study of the liver cancer gene therapy:Chitosan nanoparticles on proliferation of human hepatoma cell line SMMC-7721were observed. Different drug concentrations measured by MTT assay24,48, and72h, the growth inhibitory effect on SMMC-7721cells the role of the growth curve.3) Carcinoma model in nude mice, treatment, specimen collection and testing: Observed the therapeutic effects of chitosan nanoparticles local injection of hepatocellular carcinoma in nude mice. The establishment of the nude mice liver cancer were randomly divided into3groups (n=6), A group injected with saline group, group B is empty chitosan nanoparticles group, group C liposome group, group D load genes for chitosan nano-the genes particle group.7days after dosing, complete resection of the tumor, measuring tumor size, weight, calculate the weight of tumor inhibitory rate. Biopsy of tumor pathological changes.Results:Load gene Malay chitosan nanoparticle surface is smooth, with regular spherical morphology, particle size of150nm. The release of genes showing an increasing trend with increasing time, the release of2,4,6,8d were20%,50%,80%,90%,92%, showed the release characteristics. Malay chitosan load gene inhibition of SMMC-7721cells was stronger than the group of liposomal transfection. Chitosan weight of tumor inhibition rate of56.1%,33.7%in the liposome group, chitosan blank particles was10.2%.Conclusion:Chitosan nanoparticles on human hepatoma cell proliferation was inhibited. Chitosan nanoparticles in vitro can effectively inhibit the proliferation of hepatoma cells, the nanoparticles have good biocompatibility. Hepatocellular carcinoma in nude mice receiving nanoparticle treatment, the tumor weight and volume were suppressed. Chitosan by local injection to nude mice liver cancer can be effectively transfected into tumor cells, play an effective role in tumor suppression.