| Objects: To determine whether cultured and expanded bone marrowmesenchymal stem cells (BMSCs) in combination with nano-hydroxyapatite/zeincomplex biological materials can repair skull defects in rabbits or not.Methods: The rabbit(aged from2weeks to8weeks old) BMSCs were used asseeded cells in this study, three bone standard defects(diameter1cm)were made oneach rabbits’s skull by wheel brace drill.In group A:BMSCs were isolated andexpanded in vitro and then implanted onto the pre-molded porousnano-hydroxyapatite/zein complex biological materials,the BMSCs-nano-hydroxyapatite/zein complex biological materials were implanted into defects.Ingroup B:the defects were repaired only with nano-hydroxyapatite/zein complexbiological materials.In group C:the defects were left empty.In4,8,and12weekspostoperatively,3DCT scan and histological staining were applied.Results: In4weeks postoperatively, there were no significant difference all groupsof the area of bone defects(p>0.05).In8weeks and12weeks postoperatively,the areaof bone defect in group C were significantly smaller than group A and group B（p<0.05）,but there were no significant difference between group A and group B(p＞0.05).Results of histological examination: the gradual degradation of in material,fibrousconnective tissue hyperplasia,a small amount of lymphocytes, osteoblasts of the defectedge of hyperplasia were observed in group A and group B.Fibrous connective tissuehyperplasia, osteoblasts of the defect edge hyperplasia were observed in groupC.According Lane-Sandhu score, The results proved that the situation of bone defectsrepaire in three groups were no significant difference (p>0.05).Conclusions: The experimental study showed that the Nano-hydroxyapatite/zeincomposite was safe,biocompatible and degradable materials．The osteogenesisinductive function of the composite was limited,so it was very important to improve themechanical strength and stability of the material,or to implant a sustained releasecarrier of drug to induce osteogenesis. |
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