The Study On Effect Of Low-Level-Laser-Therapy And PRF On MG-63

Nowadays, Oral implant prosthesis technology has become a commonpractice for the patients who have dentition defect. The most difficult problemwe have to face with is the bone defection and bone deficiency of the implantarea. In order to solve the clinical problem, we carried out a lot of boneincremental technology, which can expand the operation condition, reduce thebone healing time and postoperative infection. Generally, it takes around6¹2months to bone-healing after operation. The osteogenesis quality is also asignificant factor. Therefore, how to utilize the artificial methods to increase thespeed and quality of osteogenesis has become a hot problem in the world. Low-Level-the Laser is a group of output weak laster which is lower than250milliwatts and has the biological stimulation effect. The research has provedthat it can reduce the inflammation, promote tissue repair and analgesia. It has been successfully applied to clinical medicine and oral medicine in some areas.The non-invasive and non-contact treatment with the advantage of low outputenergy and no subsidiary-injury, is called as Low-Level-Laser-Therapy. Theresearch showed that, low-level-laser can promote the bone formation, it playsa important role on the increasing of osteogenesis cells and the healing of bonedamage. Choukroun’s PRF takes these characteristics, for example, thepreparation process is simple, it completely takes from autologous blood,without adding any biological agents. It avoids the ethical controversies and therisk of cross infection, and has been successfully applied in clinical. It lasts theadvantages of platelet concentrate products PRP. Choukroun’s PRF showed asa platelet gel, contain high concentrations of platelets, variety of growth factors,and fibrinogen. The variety of growth factors play a coordination role, whichcan promote bone restoration and soft tissue regeneration.The experiment tries to put LLLT and PRF play together by two differentmethods. We prove the ability of LLLT and PRF promoting the MG-63cells proliferation and differentiation in vitro, and discuss the synergistic effect ofcombined application and single condition for the MG-63, and which providetheoretical basis for its application in the oral implant clinical areas.Methods:Put MG-63cells in10%FBS high sugar DMEM medium to culture.Take the logarithm time cells, vaccinate into different orifice. Randomize to thecontrol group （C）, the experimental group1（L1）, experimental group2（L2）and experimental group3（L3）. Extracting a26year-old male volunteersvenous blood, and quantify the blood volume, centrifuge into equivalent richplatelet fibrin membranes （PRF）. We don’t do any dispose with group C, groupL1is added the PRF, group L2is irradiated the low energy laser with the3.75J·cm^-2energy density, group L3is deal with the low energy and PRF. Collect1,3,5days the cell supernatant fluid to cryopreserve respectively.1. Detect the proliferation of MG-63at the1,2,3,5, and7days after inoculation.2. Detect the alkaline phosphatase secretion level by ALP kit at the1,3,5days.3. Detect the growth factor VEGF and TGF secretion levels of each groupof MG-63cells at the1,3,5days.Result:1. The LLLT with the3.75J·cm-2energy density and PRF has an obviouspromoting effect, there is synergistic effect after the combine application,particularly in the first5days.2. Compared with group C, the LLLT with the3.75J·cm-2energy densityand PRF has a promoting effect for the alkaline phosphatase secretion. There isobvious synergistic effect after the combine application. There is significantdifference according to compare the Absorb light luminosity values of theexperimental group and control group. The L3group is higher than group L1and L2. 3. The LLLT with the3.75J·cm-2energy density and PRF combine effectof the MG-63, the level of VEGF and TGF-β has an obvious improvement. Theconcentration of VEGF and TGF-β of the Experimental group is higher than thecontrol group C in each experimental point. The L3group is higher than groupL1and L2（P<0.05）.Conclusion：Combined application with LLLT and PRF, can take a synergisticacceleration for the proliferation, differentiation, and secretion of VEGF andTGF-β. Combined application with LLLT and PRF on the bone integration andreconstruction after oral implant postoperation, can promote the growth ofvascular endothelial, reduce the time of bone formation, improve the quality ofosteogenesis, and shorten the treatment of oral implant prosthodontics. Thisexperiment provide the theoretical basis for the combine application of LLLTwith PRF in clinical, which can be a new method for the tissue-engineered bone vascularized.