Effect Of Platelet-rich Fibrin Releasing TGF-β₁ On The Proliferation Of BMSCs In Vitro

Objective: Research that to activated platele-rich fibrin release TGF-β1 on the proliferation of BMSCs in vitro,explore its role on process of repair the alveolar bone defect. Methods:Blood was taken from central artery of rabbits,to prepare PRF by centrifugal,add them to fresh DMEM,the time points to collect exudates was in day 7,14, 21, 28 at 37 ℃. Detecting the mass concentrations of transforming growth factor-β1(TGF-β1) in the exudates of PRF.Chose the best concentration of exudates,its will be diluted to 1 ng/L,50 ng/L,100 ng/L and 150 ng/L.Let their and original concentration of exudates into conditioned medium respectively.Extract the rabbit bone marrow to culture cell.Then made the conditioned medium by exudate of PRF was used to culture BMSCs,observe its effect on proliferation of BMSCs.Results:concentration of TGF-β1 increased with time increasing, fastest growing stage of concentration was 21-28 days,at its peak to 28 days. Under the same stimulus concentration, BMSCs in 0-3 days proliferation degree is reduce in 0-1 day, increased significantly in 1-2 days, The fastest proliferation of BMSCs was 150 pg/ml group. entered into a steady phase in 2-3 days. At the same time,group of 2 days and 3 days have appeared that proliferation of BMSCs increasing with of the concentration rising, the stimulus concentration of 150 pg/ml is reach the peak, then descend. Conclusion:With the increase of time, the concentration of TGF-β1 increased gradually. The conditioned medium contains different concentration of TGF-β1 have plays a different role in promoting to proliferate of BMSCs. To cultivate the BMSCs in conditioned medium that contains 150 pg/ml mass concentration of TGF-β1,in 2-3 days,BMSCs proliferation rate is the fastest.