Expression Of CD44v6and The Relationship To Clinical And Pathological Factors In Breast Invasive Ductal Carcinoma

Background: Breast cancer is the second most common type of cancerworldwide, and is the most frequently diagnosed malignant neoplasia infamale. Breast cancer ranks second in cancer deaths in famale except forlung cancer. Invasive ductal carcinoma (IDC) is the most common type ofinvasive breast carcinoma, accounting for70%~75%of invasive breastcancer. Adhesion molecule CD44and its viriants have been consideredhaving relationship to tumorigenesis, proliferation, metastasis andprognosis. Many literatures suggest that the expression of CD44v6isassociated with the differentiation and closely related to the prognosis ofbreast cancer. However the reports are conflict. The expression ofwell-known molecular markers of breast cancer such as ER, PR, HER-2,Ki-67and p53are significantly associated with clinic, and play crucialroles in endocrine treatment, targeted therapy and prognosis prediction ofbreast cancer.Objective：This paper aims at detecting the expression of CD44v6in IDCand studying the relationship between it with the clinical pathologicalfactors. Also this paper discusses the clinical significance of the clinicalpathological detection of CD44v6in breast cancer.Methods: Collect and retrospective analysis of the relevant clinical and pathological data of37IDC cases who had accepted surgical treatment atChina-Japan union hospital of Jilin university in2011-2012. By usingRT-polymerase chain reaction (PCR) and SP immunohistochemistrymethod, we detected CD44v6mRNA levels and CD44v6proteinexpression in37IDC cases and15cases of juxtacancerous benign tissue.In addition, by using SP immunohistochemistry method, we also detectedthe protein expression of ER、PR、HER–2、p53、Ki-67、EGFR、E-cadherin.Results: The expression of CD44v6was significantly increased in IDC.At the RNA level, the expression of CD44v6was37.8%(14/37),15casesof juxtacancerous benign tissue have no expression (P=0.005). At theprotein level,32cases existed CD44v6overexpression (A total of37primary human breast IDC samples), and positive rate reached86.5%(32/37), juxtacancerous benign tissues have no expression, There was asignificant difference between carcinoma and normal breast tissue(p=0.000). The level of CD44v6mRNA and the expression of protein inIDC had no correlation with Patient’s age,tumor size, pathologicalnuclear grade, pTNM stage, axillary lymph node metastases, expressionof ER、PR and HER-2, proto-oncogene p53and the Ki-67levels reflectedcell proliferation.Conclusion: CD44v6have certain significance to identify invasive ductalcarcinoma and benign diseases. Of course, a long-term follow-up results will have more support basis. However CD44v6may not be aindependent prognostic factor in IDC. Along the researches were deeper,the role of CD44v6in IDC progress will be more clear.