| Objective:Cerebrovascular disease has become one of the major diseases which harm the elderlyhealth and life. In view of its incidence and mutilation rate is so high, prevention is veryimportant. Atherosclerosis is an inflammatory disease, CD4~+CD25~+Treg is a negativeregulator cell, can obvious against atherosclerosis. In this study we research the expressionlevels of peripheral blood CD4~+CD25~+Treg the flow cytometric, which in patients ofdifferent properties carotid atherosclerosis plaque and different intima-media thickness.Then analysis the correlation of CD4~+CD25~+Treg and blood pressure, blood glucose, bloodlipid, blood uric acid and other factors. Explore the pathogenesis of atherosclerosis, thenprovide theoretical basis for cerebrovascular disease prevention and the new treatmenttargets.Methods:We selected68patients of Affiliated Hospital of Tai Shan Medical College neurologyfrom July,2011to February,2012. Among which, there were38male cases and30femalecases. The above patients were detected of carotid IMT and plaque (including the location,number, size, echo properties) with color Doppler ultrasonic diagnostic apparatus.According to the nature of the plaque echogenicity and intima-media thickness group. Thecarotid artery atheromatous plaque strong echo in22cases, mixed echo23cases,hypoechoic23cases. Selected another21healthy cases as normal control. Theexperimental group and the control group with the nation and sex ratio no difference, agedifference were no more than5years, and in the same age group. All candidates underwentbrain magnetic resonance, blood routine, blood pressure, blood glucose, blood lipid, liverand kidney function, blood biochemical analysis, blood uric acid inspection, and clearedthe general clinical data of all cases. Determine CD4~+CD25~+Treg expression of peripheralblood with flow cytometry, then discussed the role of CD4~+CD25~+Treg in the occurrenceand development of AS and plaque stability. Application of SPSS13.0statistical software for data analysis and processing, with P≤0.05was statistically significant difference.Results:1.Comparison of peripheral blood CD4~+CD25~+Treg expression levels of differentcarotid IMT:0.9≤IMT<1.0group,1.0≤IMT<1.1group,1.1≤IMT<1.2group were lowin comparison with the control group, the difference was statistically significant (P<0.05);1.1<IMT<1.2group were lower than0.9≤IMT<1.0group and1.0≤IMT<1.1group, thedifference was statistically significant (P<0.05); Contrast between the groups of0.9<IMT<1.0and1.0≤IMT<1.1, the difference was not statistically significant (P>0.05);2.Comparison of peripheral blood CD4~+CD25~+Treg expression levels of differentnature of CAS plaques in patients: compared with the control group, group of high echo,mixed echo, low echo of subgroups were lower than control group, the difference wasstatistically significant(P<0.05); low echo group was lower than strong echo group andmixed echo group, the difference was statistically significant(P<0.05).3.Level of peripheral blood CD4~+CD25~+Treg expression of CAS patients with stableplaque group was significantly higher than that of unstable plaque group, the differencewas statistically significant(P<0.05).4.The peripheral blood expression CD4~+CD25~+Treg level in patients with CAS andLDL level are negative correlated (P=0, r=-0.523); The CD4~+CD25~+Treg level and HDLlevel are positively correlated (P=0.014, r=0.303); The CD4~+CD25~+Treg level and LPalevel are negative correlated (P=0.002, r=-0.380); The peripheral blood CD4~+CD25~+Tregexpression level and plasma triglyceride and total cholesterol level are no significantcorrelated.5.Levels of CD4~+CD25~+Treg and levels of blood glucose and blood pressure are nosignificant correlated.6.Levels of CD4~+CD25~+Treg expression of peripheral blood and high densitylipoprotein levels were positively correlated (P=0.014, r=0.303).Conclusion:1.CD4~+CD25~+Treg has the effect of stabilizing atherosclerotic plaques, levels ofCD4~+CD25~+Treg may be the degree of CAS and plaque stability of potential markers.Providing a new theoretical basis for prevention and treatment of cerebrovascular diseases.2.CD4~+CD25~+Treg and LDL, HDL, LP-a, UA interact with each other, common in theCAS occurrence, development and atheromatous plaque formation process. |
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