Studies On Chemical Constituents Of Arnebia And Composition Comparison Of The Radix And The Stem Residue

Arnebia euchroma (Royle) Johnst., and name Lithosperman euchroma Royl e, belongs to families of soft borage perennial herb.There are many researches of components and pharmacological activities of Arnebia euchroma (Royle) Joh nst.Marry compounds were isolated from Arnebia euchroma (Royle) Johnst.,such as Naphthalene quinones.Single sesquiterpanes benzene quinonoids,phenol and phenolic acids and polysaccharides.and so on.Modern efficacy studies have sho wn that these components in the main is anti-inflammatory.antiseptic.antisterility function on the cardiovascular system function.antitumor function,etc.Components of Arnebia euchroma (Royle) Johnst.were extractedn separated and identified.According to the different extraction screening.sure to petroleum ether reflux extraction the effective ingredients,and an orthogonal design was ad opted.Multi-chromatographic methods including silicagel column chromatograph.p reparative TLC and Reverse phase (RP-18)column chromatograph were used to isolate the extracts from Arnebia euchroma (Royle) Johnst.to get the monomer ic compounds.The chemical structures of the extracts were identified based on t he spectroscopic data.There are seven chemical compounds extracted including deoxyshikonin^acetylshikoninn、β/β-dimethylacrylakamin、shikonin、isobutyrylshiko nin、isobutylshikonin and acetoxyisovalerylshikonin.At the same time, compared the composition and content of Arnebia radix and the St em residues TLC and HPLC fingerprint were used to identify Arnebia,ultraviolet-visible s pectrophotometry to determine the content of hydroxy naphthoquinone total pigment in Arn ebia. HPLC to determine the total content of β-β’-dimethyl acrylamide Aka Ning and shik onin.The number of spots of Arnebia radix consistent with the Stem residues in10batche s of medicinal materials, the former was larger and deeper in color.Their features of finger print are the same,in the same retention time, the peak area of radix was larger; In10b atches of medicinal materials.the average content of hydroxy naphthoquinone total rpigment was respectively3.631percent.and1.516percent, and the total content of β-β’-dimethyl acr ylamide AkaNing and shikonin were respectively0.89570percent and0.309085percent.Th e ingredients in the radix and the Stem residues were the same, But the root of the total pigment content of hydroxyl-naphthoquinone, β-β’-dimethyl acrylamide Aka Ning and shiko nin were higher than the total content of more than twice as part of the stem residues.