Study On The Extraction Of Vitamin B₁Vitamin B₂and Preparation On Dietary Fiber From Wheat Bran

Wheat bran a byproduct of the malting process is generally used as some low value-added application such as animal feed, fermentation and so on. Wheat bran contains higher nutritional value. If developed in a reasonable way the wheat bran can be a renewable resource.This paper is studied on the extraction of vitamin B1and vitamin B2from wheat bran. Using the response surface method to optimize the extraction process of the water bath and ultrasonic assisted to extract. Obtain the optimized conditions, and preparing dietary fiber. The results of study are as follow:(1) HPLC analytical method is developed for determination of vitamin B1and vitamin B2from wheat bran According to the properties of vitamin B1and vitamin B2in structure and character, HPLC analytical method is developed for determination of vitamin B1and vitamin B2from wheat bran. Mobile phase and mobile phase concentration on retention behaviors of vitamin B1and vitamin B2were discussed. The optimal chromatograghic conditions included Hypersil C18column(150×4.6mm,5μm)and the mobile phase consisting of a mixture of0.1%acetic solution-methanol(80:20,volumn ratio) at a flow rate of1.0mL/min at25℃. The UV detection wavelength was246m. A good linear relationship was shows in the range of0.1～50μg for vitaminB1and vitaminB2, with correlative coefficient0.9938and0.9972. The simple, rapid, sensitive and accurate method could be used in quantitative determination of vitamin B1and vitamin B2in wheat bran.(2) Study on processing of the extraction vitamin B1and vitamin B2from wheat bran by the methed of water bath The extraction yield of the vitamin B1and vitamin B2as the basis, response surface methodology was respectively used to evaluate the effects of HCI concentration, temperature and time on vitamin B1and vitamin B2extraction yield from wheat bran. The optimized conditions are as follows:HCI concentration of0.6mol/L,temperature of70℃,time of65min. Under the optimum conditions, the vitamin B1and vitamin B2’s yield was10.86μg/g and7.05μg/g.(3) Study on processing of the extraction vitamin B1and vitamin B2from wheat bran by the ultrasonic assisted to extract The extraction yield of the vitamin B1and vitamin B2as the basis, response surface methodology was respectively used to evaluate the effects of solid/liquid ratios, ultrasonic power and ultrasonic time on vitamin BⅠ and vitamin B2extraction yield from wheat bran. The optimized conditions are as follows:solid/liquid ratios of100mL, ultrasonic time of20min, ultrasonic power of1000w. Under the optimum conditions, the vitamin B1and vitamin B2’s yield was12.25μg/g and7.92μg/g.(4) Study on preparing dietary fiber The residue was preparation for dietary fiber after extraction of vitamin B1and vitamin B2by a-amylase. The extraction yield of the dietary fiber as the basis, orthogonal design L9(34) was respectively used to evaluate the effects of pH, amounts of a-amylase, temperature and time on dietary fiber extraction yield. The optimized conditions are as follows:pH of8, amounts of a-amylase of0.5g, temperature of70℃, time of200min. Under the optimum conditions, the dietary fiber yield was80.5%. The WHC and SWC of dietary fiber were achieved4.1386g/g and4mL/g.