Comparative Study On Chemical Components And Pharmacological Activities Wild And Cultivated Lampteromyces Japonicus

This paper make a comparative studied on chemical composition, antitumor, antibacterial activity and antioxidant ability of wild and cultivated lampteromyces japonicus.The biological characteristics, through the medium of further optimization respectively, cultivated lampteromyces japonicus, succeeded out of lampteromyces japonicus and liquid fermentation fungus ball of Lampteromyces japonicus, get the best medium and the optimal cultivation condition. I have succeeded in cultivation, culture medium for: sawdust77%, rice bran20%, gypsum1%, glucose1%, K2HPO41%, among them dry material:water=1.0:1.2. Using the medium cultivation, the optimum temperature for15-18℃, relative humidity in80-90%. Keep light indoor and proper ventilation has scattering, in such conditions need to train180d to grow the mushroom. Liquid fermentation aspect, to lampteromyces japonicus gas bath wave bed raise, and the optimal fermentation cultivation screening formula and fermentation technology:the bacteria in the fermentation process in PD culture medium basis to join B1(1%) and chloramphenicol (50mg/mL); The optimum temperature for28℃, wave bed turn number is160RPM,training for15d, get training products, get the dry weight of2.533g/100mL.Between chemical composition, through to the TLC identification results showed that cultivated lampteromyces japonicus ethyl acetate layer in Rf=0.422place has an obvious orange spots and Rf=0.484place has an obvious green green spots, with identification meaning. Through the chemical inspection general infrared map showed that, in the functional area(1300～4000cm-1), in the fingerprint area (1300～910cm-1) and in the fragrant area (910～650cm-1) four sample all have the same absorption peaks, there are different from other samples of strong absorption peaks. This method to identify cultivated lampteromyces japonicus in infrared has auxiliary significance. Through the chemical inspection general petroleum ether extract GC-MS total ion flow chart, wild lampteromyces japonicus petroleum ether extract moonlit night mainly identified six compounds, cultivated lampteromyces japonicus petroleum ether extract moonlit bacteria mainly identified seven compounds, t succeeded out of lampteromyces japonicus petroleum ether extract mainly identified10compounds and Liquid fermentation fungus ball of Lampteromyces japonicus petroleum ether extract mainly identified nine compounds. One hexadecanoic acid methyl ester,(Z, Z)-9,12-18(2ene acid methyl ester,11-18carbon ene acid methyl ester,18methyl carbonate is four of the chemical composition.Pharmacology activity research, this article for the first time measured succeeded out of lampteromyces japonicus LD50value and succeeded out of lampteromyces japonicus ethyl acetate layer extracts antitumor activity in the body of research. The results show that high dose group (1000mg/mL) of the tumor inhibiting rates69%, low dose group (500mg/mL) of tumor inhibiting rates59%. Experiments show that succeeded out of lampteromyces japonicus with toxic ingredients reduce toxicity, but still has the remarkable for tumor inhibition.In vitro antibacterial, gram-positive and gram-negative bacteria as selected as an example, the test of wild and cultivated lampteromyces japonicus of the organic solvent extract the bacteria on the antibacterial activity experiment contrast. Take scraps of paper AGAR diffusion method respectively on wild lampteromyces japonicus, cultivated lampteromyces japonicus, succeeded out of lampteromyces japonicus and liquid fermentation fungus ball of Lampteromyces japonicus petroleum ether layer small polarity extract, chloroform layer extracts, ethyl acetate extract of medium polarity, acetone layer extracts, methanol extracts of large polarity in inhibit the growth of the bacteria. The results show that the three samples of e. coli ATCC8099chloroform group the inhibition rate and staphylococcus aureus ATCC6538antibacterial activity is the most significant effect, and as the subjects of the drug concentration increases, antibacterial effect also increases, showed a quantity-efficiency relation.In the antioxidation experiment, in vitro1,1-diphenyl-2-picrylhydrazyl radical (-DPPH) and superoxide anion radical (·O2-) test systems were established to study wild lampteromyces japonicus cultivated lampteromyces japonicus and succeeded out of lampteromyces japonicus of petroleum ether extract succeeded, chloroform extract, ethyl acetate extract, acetone extract, methanol extracts, the oxidation of evaluation. The results show that the three samples of DPPH free radicals and hydroxyl radicals are has the very high clear role.