The Detection And Significance Of Antiapoptotic Protein BCL-XL In Immune Thrombocytopenia(ITP)

Objective:To observation the expression level of antiapoptotic protein BCL-XL with immune thrombocytopenia patients and health persons’peripheral blood platelets and lymphocytes,to understand the role of BCL-XL in ITP, try to provide new basis for the diagnosis and treatment of ITP.Methods:The flow cytometry was used to analyze the expression of BCL-XL in28cases of patients with ITP and15cases of health persons’ platelets and lymphocytes. According to platelet counts up to50×109/L above, haemorrhage symptom disappeared as a treatment for a reaction standard with the ITP after glucocorticoid treatment divided into the effective group and the invalid group. And the effective group will be according to the platelet increased degree divided into A and B two subgroups, after treatment the platelet rose to95×109/L above is group A, after treatment the platelet rose to>50×109/L but<95×109/L is group B. All ITP before the treatment and after two weeks glucocorticoid treatment the extraction of peripheral blood, detection BCL-XL expression in lymphocytes and platelets.Results:1. The antiapoptotic protein BCL-XL expression rate in the platelets of ITP patients statistically reduced than normal (t=4.12, P<0.001), and in the lymphocytes were significantly increased (t=7.94, P<0.001).2. Compared with the health group, the BCL-XL expression rate of ITP patients after treatment with effective group in the platelets is somewhat increased, in the lymphocytes reduced but have no difference(P>0.05);The BCL-XL expression rate of ITP patients after treatment with invalid group in the platelets is significantly less, in the lymphocytes statistically increase(P<0.01).In the ITP patients with after treatment, the BCL-XL expression rate in the platelets of invalid group obviously lower than effective group, in the lymphocytes of invalid group obviously higher than effective group (P<0.01).3. The BCL-XL expression rate of ITP patients with effective group in platelets after treatment is obviously higher than before treatment(their matching t test, n=19, t=2.48, P<0.05);But if the effective group according to the platelet rise extent divided into A and B two subgroups, A group after treatment the platelet rose to95×109/L above, a total of10example, its BCL-XL expression in the platelets rising somewhat higher than before treatment (t=2.80, P<0.05), and after treatment the platelets of B group rose to>50x109/L but<95×109/L, total of9cases, the BCL-XL expression in the platelets of before and after treatment has no difference (t=0.84, P>0.05), but because observation cases, has remains to be accumulating material. The BCL-XL expression rate of ITP patients with effective group whether A or B group in lymphocytes after treatment is obviously lower than before treatment, and both are P<0.001.4. The BCL-XL expression rate of ITP patients with invalid group after treatment is somewhat higher in platelets than before treatment, in the lymphocytes is reduced, but there were no significant difference (P>0.05).Conclusion:1. The BCL-XL with ITP patients in the platelets existing lower expression, after glucocorticoid treatment the effective group high expression, hints in ITP patients existing platelet cell apoptosis phenomenon, the platelet abnormalities apoptosis may be one of the mechanism of ITP.2. The BCL-XL with ITP patients in the lymphocyte existing high expression, after treatment the effective group lower expression, showed that insufficient lymphocytes apoptosis existing in ITP, lymphocytes apoptosis reduce may be involved in the pathogenesis of ITP.