Significance Expression Of Osteopontin In CIN And Carcinoma Of Cervical

ObjectiveTo investigate development role of osteopontin(OPN) in cervicalintraepithelial neoplasia and cervical cancer.MethodsThe cervix specimens were collected from the Pathology Department of thefirst affiliated hospital in Liaoning Medical College, among them there were50cases of cervicitis,57cases of CINⅠ,52case of CINⅡ,48cases of CINⅢ,62cases of cervical squamous cell carcinoma. Another five cervicitis, CINⅠ,CINⅡ, CINⅢ, and cervical squamous carcinoma fresh specimen were collected.Immunohistochemical method and westerblot method was performedrespectively to detect the expression of OPN, the data was compared andanalyzed to reveal the difference and correlation of express of OPN in benigntissue and cancer tissue. Meanwhile the relationship of OPN expression andhistologic classification, lymphatic metastasis, TNM phase, age factor wasdiscussed in squamous carcinoma of the cervix.ResultsThe OPN expressed in all the different tissues above, it located mainly onthe membrane and cytoplasm. But The OPN expression ratio and intensionwere different. In cervicitis, CINⅠ, CINⅡ. CINⅢ and cervical squamous cellcarcinoma the positive expression rate was6.0%（3/50）,10.5%(6/57),15.4（8/52）,22.9%(11/48) and83.8%(52/62) respectively. The OPN expression ofCINⅢgroup was different with the CINⅡ, CINⅠand cervicitis group（P<0.05）; carcinoma of the cervix group was significance different with the other group（P<0.01）; The cervicitis group, CINⅠand CINⅡgroup the OPN expressionwas no different（P﹥0.05）. Westernblot analysis showed the OPN expressionwas significantly higher in the squamous carcinoma of the cervix than the othertissues（P<0.01）. CINⅢ was different with the CINⅡ, CINⅠand cervicitis group（P<0.05）; it had no statistics distinction in cervicitis group, CINⅠandCINⅡgroup（P﹥0.05）. The OPN had no relationship with age（P﹥0.05）; It hadrelativity with the histologic classification, lymphatic metastasis and TNM phase（P<0.05）.ConclusionsThe over expression of OPN may promote the malignant progress incervical cancer, and facilitate it invasionbility.