Study On The Effects And Mechanisms Of Pseudolaric Acid B On Apoptosis In Human Bladder Cancer5637cells

ObjectiveTo investigate the apoptosis of bladder cancer cell5637induced by pseudo-laric acid B in vitro and further its mechanism．Aims to open up new plants inthe multimodal treatment of bladder cancer chemotherapy to provideexperimental data.MethodsThe cell proliferation was detected by MTT assay；the cell apoptosis wasobserved by inverted phase contrast microscope and flow cytometry withAnnexin V-FITC/PI double staining；the expressions of survivin protein andcaspase-3protein were detected by Western blot assay.ResultsIt showed that pseudolaric acid B remarkably induced apoptosis of5637cell line and the inhibition was depended on the exposure dose and time.IC50is165.91±20.75μmol·L^-1、89.85±13.43μmol·L^-1、48.31±2.54μmol·L^-1when treatedwith PBA for24、48、72hours.Moreover, pseudolaric acid B suppressed survivinand up-regulated caspase-3expression.Conclusionspseudolaric acid B inhibits the proliferation and induces the apoptosis of5637cells.The molecular mechanism of pseudolaric acid B inducing theapoptosis of5637cells may be associated with its action of down-regulating theexpression of survivin，and up-regulating the expression of caspase-3.