| Objective1. To detect the expression of MTDH and HIF-1α with the pathologicaltissues in metastasis breast cancer.2. To detect the expression of MTDH and HIF-1α with the peripheral bloodin metastasis breast cancer.3. To study on the relationship among MTDH, HIF-1α and clinicalinformation of breast cancer.4. To study on the relationship between MTDH and HIF-1α in breast cancer.5. To study effects of MTDH and HIF-1α on the Neoplasm Metastasis ofbreast cancer.Methods1. Cellular immunochemical was used to detect the expression of MTDHand HIF-1α protein in breast cancer cell line MCF-7, breast cancer cell lineMCF-7and MDA-MB-231.2. Immunohistochemical assay was used to detect the expression of MTDHand HIF-1α protein in cancerous tissues from21metastasis breast cancerpatients and9primary breast cancer, and we compared the expression ofMTDH and HIF-1α.3. FCM was used to detect the expression of MTDH and HIF-1α protein inbreast cancer cell line MCF-7, breast cancer cell line MCF-7and MDA-MB-231.4. FCM was used to detect the expression of MTDH and HIF-1α protein in the peripheral blood from21metastasis breast cancer patients and9primarybreast cancer.Results1. MTDH was expressed in the cellular membrane and the cytoplasm ofbreast cancer cells, and only a few of MTDH of pathological tissues were in thecell nucleus. HIF-1α was expressed in the cytoplasm and the cellular nucleus.2. In immunohistochemical study, the positive rates of MTDH in breastcancer patients, metastasis breast cancer patients and primary breast cancerpatients were63.3%(19/30),73.8%(15/21) and38.9%(4/9), and the positiverates of HIF-1αin breast cancer patients, metastasis breast cancer patients andprimary breast cancer patients were23.3%(7/30),23.8%(5/21) and22.2%(2/9).3. Correlational analysis of the expression of MTDH in breast cancer andthe clinical pathological parameters showed that obvious correlation existedbetween the clinical stage(P=0.010), the metastatic sites(P=0.003), time toprogression(P=0.002), Ki67(P=0.029), P53(P=0.022) and histologicaltype(P=0.023), although patients age(P=0.970), lymph nodemetastasis(P=0.694), ER(P=0.056), PR(P=0.428) and HER-2(P=0.282) did notseem to correlate. The expression of HIF-1α had no correlation with the clinicalpathological parameters of the breast cancer patients.4. Results by FCM, positive rate of MTDH and HIF-1a were (7.44±6.02)%,(37.06±24.77)%, respectively. There was statistic significance in research onLinear Regression because of the regression coefficient (R=0.365, P<0.05).Conclusions1. Both MTDH and HIF-1α were expressed in the cancerous tissues andperipheral blood from the patient of breast cancer.2. The positive rate of MTDH and HIF-1α in metastasis breast cancer wassignificantly higher than primary breast cancer, detecting out from the cancerous tissues and peripheral blood.3. Significant correlation was found between the MTDH and HIF-1α for themetastasis and the angiogenesis of breast cancer. MTDH gene and HIF-1αgene play important roles in breast cancer. |
