The Expression Of Sox2、C-Myc And Her2in Gastric Carcinoma Tissues And Their Clinical Significance

Background and purpose:Gastric carcinoma is one of the most common malignant tumors in the world and the mortality of it is very high, but the diagnose ratio of early stage is so low. The patients become younger and younger with more severe maliganancy in China nowdays. In this study, we detected the expression of SOX2, C-MYC,HER2in130gastric carcinoma cases and assessed their clinical significance.Materials and methods:130samples of gastric carcinoma which were excised by gastroectomy and20samples of the corresponding normal gastric mucosa tissues as normal controls. We performed tissue microarray and PV-9000two-step immunohistochemical staining methods to detect the expression of SOX2, C-MYC and HER2in the gastric carcinoma tissues and normal gastric mucosa tissues. Then we analyzed the expression of the three genes with cliniopathological characters in gastric carcinoma tissues.Results:The positive expression of SOX2, C-MYC and HER2were all located at the cytoplasm and cell membrane with brown granules.1. SOX2was positive in88cases of the130gastric carcinoma cases (67.69%) and positive in18cases of the20gastric mucosa tissues (90.00%), obviously the expression of SOX2was downregulated in gastric carcinoma tissues compared with normal mucosa.The expression of SOX2showed no significant difference in patients’ gender and age.The positive expression was significantly higher in non-mucinous adenocarcinoma than in mucinous adenocarcinoma and signet ring cell carcinoma. The tumors with poorer differentiation,deeper invasion,later TNM stage and lymph node metastasis were accompanied with lower SOX2expression.The association in each group was statistically significant determined by P<0.05.2. The positive ratio of C-MYC in gastric carcinoma tissues was60.76%(79/130), but negative in all of the normal mucosa, so obvious overexpression of C-MYC in the carcinoma tissues. The expression of C-MYC showed no obvious difference in the groups of patients’ age, gender and tumor differentiation.The positive expression was significantly higher in non-mucinous adenocarcinoma (66.34%) than in mucinous adenocarcinoma(38.46%). The higher expression of C-MYC had association with deeper invasion,lymph node metastasis and later TNM stage. The association in each group was statistically significant determined by P<0.05.3. Positive rate of HER2was36.15%(47/130) in gastric carcinoma cases, and with negative expression in all of the normal mucosa, so obvious overexpression of HER2in the carcinoma tissues.Showing no significant difference in groups of patients’ age,gender, tumor histological type and differentiation. The tumors with deeper invasion, later TNM stage and lymph node metastasis had higher expression of HER2. The association in each group was statistically significant determined by P<0.05.4. Correlation of SOX2, C-MYC and HER2analysis in gastric carcinoma:Expression of SOX2and C-MYC were not found related; HER2expression was significantly associated with SOX2and C-MYC.Conclusions:1. All of the three genes SOX2, C-MYC and HER2are related with gastic carcino-genesis,furthermore they are all involved in the process of invasion and metastasis of gastric carcinoma, with association of TNM stage.2.The expression of SOX2and C-MYC is obviouly higher in the non-mucinous adenocarcinoma than in mucinous adenocarcinoma,which indicates that when the cancer cells produce mucin they will express SOX2and C-MYC in lower level. 3.The overexpression of HER2after been activated could make the expression of SOX2downregulated;the cowork of C-MYC and HER2is very important factor during the genesis and development of gastric carcinoma;but SOX2and C-MYC is independent.4. The detection of SOX2,C-MYC and HER2may give guides to treat gastric carcinoma.