| Objective:To observation the expression of Paired box2and WiIms’tumor1gene in Liuweidihuang Decoction on5/6nephrec-tomized rat remnant kidney, discussion of Liuweidihuang Decoction to prote-ct renal function, the mechanism of delaying kidney damage.Methods:In accordance with the completely random methods72male SD rats were divided into3groups:Black group(12rats), sham operation group(12rats), operation group,model group(48rats), operation group with5/6nephrectomy induced chronic renal failure rat model,1weeks,5rats died after surgery, In accordance with the completely random methods43male SD rats were divided into3groups:The model group(14rats), the enalapril group(14rats), Liuweidihuang Decoction group(15rats).Sham operation group undergoing operation two times, each time only open celiac, exposed kidneys, abdominal closure. Second operation after3days, the rats were treated by gavage. In eighth weeks in rats were sacrificed, observation of remnant kidney tissue morphological changes were observed with immunohistochemistry rat kidney Paired box2and Wilms’tumor1gene expression. Results:1By using HE and Masson staining with5/6nephrectomy rats renal tubular interstitial tissue morphological changes,5/6nephrectomy induced renal damage in rats model of successful re-production, Liuweidihuang Decoction and enalapril group can redu-ce renal tubulointerstitial damage and fibrosis degree;2.Immunohist-ochemical semiquantitative analysis revealed six rehmannia root gro-up of renal cortical Paired box2and Wilms’tumor1gene expression is less than the model group;3. Liuweidihuang Decoction can lower blood levels of Scr.Conclusion:Liuweidihuang Decoction may be adopted to decrease the accumulation of extracellular matrix, downregulation of Paired box2, WiIms’tumor1gene expression, make the cell matrix components decreased synthesis, in order to reduce inflammation and fibrosis, to prevent renal interstitial fibrosis, protect the kidney function. |
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