| 【Objective】To investigate the effect of saturated hydrogen saline on artery tissue remodelingand mechanical property maintaining of abdominal aortic aneurysm of rats.Furthermore, to describe whether hydrogen can be used as a new treatment to retardthe progression of AAA based on the role of inflammation and oxidative stress in thepathogenesis of AAA.【Methods】1.In healthy male Sprague-Dawley rats, AAA was induced by infiltration ofabdominal arota with0.5mol/L calcium chloride. Saturated hydrogen saline (5ml/kg)or saline was administred intraperitoneally once daily.2.Twenty-eight days later, the diameter, wall thickness and opening angle of theartery rings were mearsured to evaluate residual strain of the aorta.3.The aortic tissue was exercised for HE and elastic fiber staining to observedestruction and degradation of the structure.4.The protein expression and mRNA expression of rCKLF-1, MMP-2and MMP-9in AAA tissue were observed by immunohistochemistry staining and real-time PCR.5.Pro-inflammatory cytokines (IL-1β, TNF-α) in AAA tissue were detected withELISA.6.MDA, SOD content of samples were determined with Spectrophotometry.【Results】1.AAA can be diagnosed as abdominal aortic diameter expansion more than50%.The two group both reached the standard, aneurysm formation rate was100%. Theexpansion rates of experimental group and control group were respectively65%and128%, which were significantly different (P<0.01). 2.Compared with control group, the opening angle increased with the diameter andthe thickness of the aorta decreasing significantly (P<0.01).3.Compared with the control group, the infiltration of inflammation, the injury ofelastic fibers in the vessel wall, and the positive expression of rCKLF-1, MMP-2and9protein of the experiment group were all reduced.4.The relative mRNA expression of rCKLF-1of the experiment group was2.24±0.87, significantly lower than that of the control group (4.63±1.05, P<0.01); therelative mRNA expression of MMP-2of the experiment group was2.34±0.85,significantly lower than that of the control group (11.64±2.51, P<0.01); and therelative mRNA expression of MMP-9of the experiment group was2.87±0.55,significantly lower than that of the control group (6.66±0.89, P<0.01).5.The tissue IL-1β level of the experiment group was86.23±7.80pg/ml,significantly lower than that of the control group (139.37±6.64pg/ml, P<0.01); andthe tissue TNF-α level of the experiment group was89.50±13.33pg/ml, significantlylower than that of the control group (354.68±39.66pg/ml, P<0.01).6.The tissue MDA level of the experiment group was1.52±0.38U/mg, significantlylower than that of the control group (4.81±0.22pg/ml, P<0.01); and the tissue SODlevel of the experiment group was84.03±3.50U/ml, significantly higher than that ofthe control group (66.11±10.61U/ml, P<0.01).【Conclusion】1.Saturated hydrogen saline can maintain good mechanical properties and reducedilatation of the aorta by increasing residual strain and reducing the remodeling of it.2.Mechanisms of hydrogen inhibition of AAA formation include the followingpoints:(1) reduce expression of vascular wall protein hydrolase and degradation ofelastic fibers;(2) suppress inflammatory cells from infiltration, low the expression ofcytokines and chemokine, mitigate inflammatory injury, and improve prognosis.;(3)reduce tissue peroxidation, mobilize the endogenous anti-oxidative system. |
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