Interventional Effects And Mechanisms Of SLTN Granules On Focal Cerebral Ischemia Injury

Objective:Concept SLTN particles intervention of focal cerebral ischemic injury andexplore its intervention mechanism through SLTN particles active ingredientsOxymatrine neuroprotective effects and mechanisms to further clear at the cellularlevel, thus pharmacological experimental evidence for the brain particles preventionof cerebral ischemic injury clinical development SLTN application.Methods:In this study,150adult male healthy Wistar rats were randomly divided into sixgroups: sham group, model group, Xuesaitong dispersible tablets positive drug group(0.36g·kg^-1), SLTN particles low dose group (1.35g·kg^-1), SLTN particles middle dosegroup (2.7g·kg^-1), SLTN particles high dose group (5.4g·kg^-1), pre-administered for5days after the suture method blockage to the Preparation of the middle cerebral arterymodel of focal cerebral ischemia.24h after the determination of relevant indicators.Cerebral ischemia in rats degree of recovery of neurological function, infarct sizecalculated based on school ratings and brain tissue of nerve function observed by HEstaining method to detect pathological changes of the rat brain, SOD activity andMDA content indicators to evaluate SLTN particles of the therapeutic effect of focalcerebral ischemia, observe the intervention of focal cerebral ischemic injury. Thebrain tissue, endoplasmic reticulum stress marker protein GRP78expression detectedby immunohistochemistry and Western blot method, using immunohistochemicalmethods observed apoptotic key proteins in the endoplasmic reticulum stress CHOPand Caspase^-12expression, so as to explore the brain particles intervention effect oncerebral ischemia injury may mechanism. To further investigate the neuroprotectiveeffect and mechanism of the active ingredient particles Oxymatrine, endoplasmicreticulum stress inducer in vitro tunicamycin stimulation of PC12cells to endoplasmicreticulum stress by CCK-8assay cell viability, and thus determine the of tunicamycinconcentration and Oxymatrine optimal dose. Microscope observation of Oxymatrineon the morphological changes of the nerve cells, Oxymatrine GRP78expression was observed by Western blot.Results:The results show that SLTN particles can reduce nerve function scores in focalcerebral ischemic injury in rats, reduced infarct size of rat brain tissue, reduce ratbrain tissue MDA content and SOD activity, and improve brain missing blood damagecaused by the brain tissue lesions. Prompted SLTN, Qingnaoqranule focal cerebralischemic injury intervention. Immunohistochemistry and Western blot results showthat GRP78protein in the endoplasmic reticulum stress, SLTN particles can be raised,lowered the endoplasmic reticulum stress apoptosis-related protein CHOP andCaspase^-12expression. Intervention mechanism prompted SLTN particles of focalcerebral ischemic injury may be related to the impact endoplasmic reticulum stress.In order to the further concept SLTN the neuroprotection and mechanism of thethe brain particles active ingredient Oxymatrine, at the cellular level,20μMtunicamycin induced PC12cells endoplasmic reticulum stress given prior1mg· ml^-1Oxymatrine can ease the degree of cell damage, raised the flag of endoplasmicreticulum stress protein GRP78expression, suggesting that Oxymatrine can affect theendoplasmic reticulum stress, improve and protect nerve GRP78expression wasobserved after24h cells.Conclusion:The Oxymatrine given prophylactically SLTN particles can effectively intervenein rat focal cerebral ischemic injury, its the intervention mechanism may be related tothe endoplasmic reticulum stress, further research, SLTN found particles of the activeingredient can be by impact of endoplasmic reticulum stress protects nerve cells.