Chemical Constituents Of Three Endophytic Fungi From Bryophytes

Endophytic fungi, consisting mostly of Ascomycota, are the organisms that colonize the internal healthy tissue of symptomless plants. Endophytic fungi appear in almost all the plants on the earth. Because of the reciprocal relationship between the endphytic fungi and their host formed during the long co-evolution of them, the endophytic fungi always certain functional metabolites which can affect their host’s ecology or as pharmaceuticals. This interesting fact has aroused much attention. Many researches have been launched and resulted in the isolation of a number of metabolites with various biological activities:such as antibacterial, antifungal, anticancer, immunosuppressive, insecticidal and so on. However, studies on the endophytes associated with bryophytes, especially mosses have been rare and badly in need.In this paper, three endophytic fungi from bryophytes were fermented for their chemistry investigation.30compounds was obtained after extraction, separation with silicagel and sephadex LH-20column and HPLC, including7xanthones,8aromatics,4steroids,3alkaloids,3benzoquinones and5others.4of them were new, which were3xanthones:sydoxanthone A-B (1-2),13-Oacetylsydowinin B (3) and1aromatic N-(2-(4-hydroxyphenyl) acetyl) propionamide (13). The structures of them were conformed through spectroscopic data including UV, IR, MS, and NMR. Some of above compounds were screened for thier anticancer, antifungal and immunosuppressive activities.