The Study On Anti-radiation And Anti-oxidation Effects Of Astragalus Extracts

Objective:To study the protective effects on mice with irradiation damage and the anti-oxidation function of Astragalus extractsMethods:1The anti-radiation damage effects of Astragalus extracts160Kunming mice were randomly divided into4groups, each group was randomly divided into4subgroups, the Astragalus extracts low-dose group, moderate-dose group, high-dose group, and the model control group (10mice each group). The three dose groups were given Astragalus extracts at doses of150mg/kg.bw、300mg/kg.bw、900mg/kg.bw. The model control group was given distilled water.30days later,3groups were given the y-irradiation at the dose of4Gy, and1group7Gy. The total number of the white blood cells(WBC) in the peripheral blood was measured1day before radiation,3days and14days after radiation. The DNA content and the ratio of micronucleus cells in marrow were measured3days after the radiation The SOD activity in blood and liver were measured7days after radiation.2The anti-oxidation effects of Astragalus extracts.40Kunming mice were randomly divided into4groups, the model control group, the Astragalus extracts low-dose group, moderate-dose group, high-dose group. The three dose groups were given Astragalus extracts at doses of150mg/kg.bw、300mg/kg.bw、900mg/kg.bw. The model control group was given distilled water at the same dose.30days later, the SOD activity in erythrocytes and GSH-Px activity in blood were measured. Then0.4mg/kg.bw Bromobenzene was given.20hours later, mice were killed, then the SOD activity, GSH-Px activity and MDA content in liver were measured.Results:1The anti-radiation damage effects of Astragalus extracts At the first of the experiment, in the middle of the experiment,and at the end of the experiment, the body weight in each subgroup were not significantly different(P>0.05).1day before radiation, the model control group was compared with the low dose group, the moderate group and the high dose group, there was no significant difference(P>0.05).3days after radiation, WBC in the high dose group increased compared with the model control group(P<0.01), but there were no difference between low dose, high dose group and model control group(P>0.05). WBC of the model control group decreased significantly3days after radiation compared with1day before radiation(P<0.01),and it shows that the radiation model was successful.14days after radiation, the model control group was compared with the low dose group and moderate group, there were no significant difference(P>0.05). The WBC in high dose group increased significantly compared with model control group(P<0.01).3days after radiation were given, the DNA content in marrow of the model control group were higher compared with the three dose groups, and the difference were all significant(P<0.01).The ratio of micronucleus cells in marrow of model control group were higher compared with the three dose groups, and the difference were significant between moderate dose group, high dose group and model control group(P<0.01). The ratio of micronucleus cells in marrow of low dose group was not different with the model control group (P>0.05).7days after the y-irradiation were given, the erythrocytes SOD activity in the three dose groups were all significantly higher compared with the model control group(P<0.01).The liver SOD activity in the three dose groups were all significantly higher than the model control group(P<0.01).2The anti-oxidation effects of Astragalus extracts. The erythrocytes SOD activity in the high dose group and moderate dose group were higher compared with the model control group(P<0.01),and there was no difference between the loe dose group and model control group(P>0.05).The liver SOD activity in the three dose groups were all much higher compared with the model control group(P<0.01).The blood GSH-Px activity in low dose group, moderate dose group, and high dose group were all significantly higher compared with the model control group (P<0.05, P<0.05, P<0.01)。The liver GSH-Px activity in high dose group was significantly higher compared with the model control group(P<0.01),and there was no difference between low dose group,moderate group and the model control group(P>0.05).The MDA content in the low dose group, moderate group, and high dose group were significantly lower compared with the model control group((P<0.01)。Conclusions:1. The result of the anti-radiation damage experiment shows that the number of the white blood cells of the peripheral blood, DNA content level in marrow, the erythrocytes SOD activity and liver SOD activity in the groups given Astragalus extracts were much higher than the model control group, and the ratio of micronucleus cells in marrow was much lower than the model control group. The high dose group of Astragalus extracts have the strongest anti-radiation damage activity. It shows that the Astragalus extracts have protective effects on radiation damage.2. The result of the anti-oxidation experiment shows that the groups given Astragalus extracts have much higher erythrocytes SOD activity, liver SOD activity, blood GSH-Px activity, liver GSH-Px activity, and much lower MDA content in liver than the model control group. It shows that the Astragalus extracts have anti-oxidation function.