The Study Of HBV BCP Mutation In Tumor Tissues, Adja-cent Non-tumor Tissues And The Serum From Patients With Hepatocellular Carcinoma

Hepatocellular carcinoma is one of the most important malignant tumor in China, itsoccurrence is affacted by many factors, including virus and the genetic factors. Chronichepatitis B virus infection is the major risk factor for HCC. HBV infection could activecellular immune response, However, many sustained virus carriers still can not get rid ofthe virus. Persistent infection for many reasons, genetic variation is one of the reasons. Thevariation can occur between HBV infection pathogenic phase and antiviral therapyduration. Mutation of the virus can obtain greater virulence of the virus, liver cell invasionforce, resistance to antiviral treatment, and can change the virus surface active immuneepitope.In the clinical practice, we find the recurrence of HCC is very common. Therelationship of chronic HBV infection and post operation recurrence is largely unclear.Are the HBV variants in tumor tissue, adjacent non-tumor tissues and the serum the same?If not, can be reveal some basic rules HBV lead to the development of HCC? This studywas aimed to explore the associations of the mutions of the cancer tissue, adjacent tissueand serum of HBV-related HCC. And then provide important clues for the prevention ofthe risk factors of HCC in China. Methods: This study includes103cases of primary livercancer patients. The extract of HBV DNA and genetic sequencing were analyzed by thepolymerase chain reaction (PCR) and cloning technology. The SPSS19.0,and chisquaretestjiu90was used to analyze the genetic data.Results:1. The mutation frequency isdifferent of the1762nt.1764nt.1846in HCC tumor tissues and adjacent non tumor tissues.2. The mutation of nt.1762、 nt.1764and nt.1846in HCC tumor tissues and adjacentnontumor tissues is significant correlation.