The Clinical And Experimental Research Of Renal Pathological Physiology Change Caused By Nutcracker Syndrome

Section one Renal pathological change caused by adult simple nutcracker syndromeObjectiveTo study renal pathological changes caused by simple nutcracker syndrome.Methods18cases of adult patients suffering nutcracker syndrome and diagnosed by ultrasonography and CTA,underwent urinary sediment red blood cell morphology and count, urine, kidney function and24h urinary protein quantitative analysis. The patients of concomitant glomerulonephritis were ruled out. The reminder underwent left renal biopsy. The microstructure, immune complexes and ultrastructure of the kidney were observed by the microstructure of light microscopy, immunofluorescence and transmission electron microscopy.Results18diagnosed patients, optical microscope, bliteral kidneys, mesangial cells and mesangial matrix no significant change,endothelial cells of capillary no hyperplasia and no change in renal interstitial and tubular. Immunofluorescence (In addition to the two hepatitis B virus carriers prompted HBSAg (+)) showed no immune complex deposition. Transmission electron microscopy, cytoplasmic lysosomes increased, mitochondria mildly swelled. ConclusionThe nutcracker syndrome does not cause significant change in the left kidney substance and function, but caues cell mildly ischemic changes. Section two Effect of left renal vein compression on expression of AQP1and AQP2in rabbit kidneyObjectiveThe animal model of nutcracker syndrome was established by partial ligation of the left renal vein. A preliminary study of AQP1and AQP2expression in renal tubular provided the molecular basis of materials for left renal pathophysiological changes caused by nutcracker syndrome.METHODS:Adult New Zealand white rabbits were randomly divided into the three group: sham group(A),50%ligation group (B),75%ligation group (C), two months later, the level of expression of proteins AQP1and AQP2were detected by Western Blot in each group.ResultsThe analysis of AQP1and AQP2protein bands gray value in three groups of kidney tissue shows that:expression of AQP1in the model group compared with sham group was significantly decreased (P<0.05). Expression of AQP1in75%ligation group compared with50%ligation group and sham group also significantly reduced (P<0.05). The difference was statistically significant. The AQP2expression level have no significant changes in50%ligation group and sham group (P>0.05).AQP2expression levels of75%ligation group compared with the sham group and50%ligation group decreased significantly (P<0.05), the difference was statistically significant.ConclusionThe left renal vein hypertension make AQP1expression reduce in renal tubular and AQP1expression is probability to the degree of vascular compression. AQP2was no obvious change in the mild compression. Expression levels began to decline when vascular compression aggravates. The nutcracker syndrome can affect renal reabsorption by affecting the expression of the AQP1and AQP2.