| BackgroundSchizophrenia belongs to a kind of common psychosis. Some reseaches revealed that the lifetime prevalence rate of schizophrenia patients was about3.8%o to8.4%o around the world, and a lot of people were in the state of different degrees of schizophrenia in China. Once suffering from schizophrenia, it will affect the patient’s quality of life and health in varying degrees. The genetic factors, endocrine factors, physical biology factors, changes in brain structure and other aspects were considered to be the causes of schizophrenia. Some studies showed that the attack of schizophrenia had close relation to neural developmental anomalies, and the brain-derived neurotrophic factor (BDNF) was one of the most critical one in all factors. In this study, we intended to provide experiment data for the pathogenesis of schizophrenia through analysis the correlation between the clinical phenotype of schizophrenia patients and the polymorphisms of brain derived neurotrophic factor gene, as well as the serum BDNF concentration.MethodsSelected456cases of schizophrenia patients in inpatient psychiatric hospital as the research objects, and simultaneously selected114cases of normal persons as the experimental control group. The experimental patients group was scored with PANSS scale and PSP rating scale by the same experienced psychiatric doctor. Using PSP rating of different clinical diagnosis group classification to analysis the affect of the different clinical subtypes of schizophrenia to patients’social cognition and social functioning, enzyme-linked immunosorbent (ELISA) method to detect BDNF levels of specimen serum in the two groups, TaqMan method to detect the single nucleotide polymorphisms (SNPS) rs6265and rs11030101loci of BDNF gene. Analysis the correlation between schizophrenia patients gender, age, with the serum BDNF concentration, between different clinical diagnostic classification of schizophrenia patients with the serum BDNF concentration, between different (SNPs rs6265and rs11030101) genotype, allele distribution, joint genotype frequency distribution with the schizophrenia.ResultsPANSS score of experimental group patients showed that there was no statistical significance between I type and II type patients’sex ratio, average duration and average age (P>0.05); PSP scale average score of experimental group patients was (46.04+16.02), this revealed that patients’social cognition and social function had varying degrees of damage at the onset; PSP score showed that there was no statistical significance between different clinical classification of experimental group patients(P>0.05); the experimental group patients serum BDNF was lower than the control group and the difference had statistical significance(P<0.05); there was no statistical significance of serum BDNF levels between different gender in two groups(P>0.05); and the same to different ages in two groups, and the different clinical diagnosis in experimental group patients. The rthogonal correlation analysis of serum BDNF levels, PSP scale score and PANSS scale score in experimental group patients showed that, PSP rating and PANSS score in patients was negative correlation and the difference had statistical significance(P<0.05), the correlation between other index was no statistical significance(P>0.05); There was no statistical significance between SNPs rs6265and rs11030101genotype and allele in the two group(P>0.05); and the same to the frequency distribution of men and women in two patients groups(P>0.05); the frequency distribution of joint genotype (SNPs rs6265rs11030101) in two groups of patients was no statistical significance(P>0.05); the distribution of SNPs rs6265and rs11030101genotype and allele in different clinical diagnosis classification had no statistical significance in experimental group(.P>0.05); the PANSS score of patients with SNPs rs6265and rs11030101different genotypes in experimental group had no statistical significance(P>0.05); PSP score of patients with SNPs rs6265different genotypes had no statistical significance(P>0.05); PSP score of patients with SNPs rs11030101different genotypes showed that, G/A was the highest and A/A minimum, the difference had statistical significance(P<0.05); the serum BDNF concentration of experimental group patients with different SNPs rs6265rs11030101genotype had no statistical significance(P>0.05).ConclusionThe serum BDNF levels of schizophrenia patients is lower than normal person. Schizophrenia patients with different genotypes of SNPs rs11030101loci may affect the scores of individual and social function, and the influence of G/A type patients is the highest, the A/A type patients is the minimum... |
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