| Objective: To explore the the effects on the abutment periodontal tissuevia test the amount of gingival crevicular fluid (Gingival Crevicular Fluid,GCF)and tumor necrosis factor-α (Tumor Necrosis Factor-α, TNF-α) andinterleukin-1β (Interleukin-1β, IL-1β) concentration level at different periodsof the abutments on three different base material porcelain crown (BakedPorcelain crown), and provide some experimental evidence on clinicalmaterial selection and clinical evaluation.Methods:1Case selection and experimental groups respectively: Patientswith Co-Cr alloy porcelain crown, titanium porcelain crown and zirconiaall-ceramic crown maxillary anterior teeth of30cases (including9male cases,21female, mean age27.7years) periodontal health, at least three months nottaken antibiotics, no systemic diseases, no pregnancy in female patients. Instrictly limited contour, marginal fit, periodontal status and edge position andother aspects of the case, the selection of standard PFM crown30, each kindof porcelain fused to metal crown each10, divided into A, B, C three groups,namely the Co-Cr alloy porcelain crown group (A group), pure titaniumporcelain crown group (B group), zirconia all-ceramic crown group (Cgroup), to repair the former as control.2Testing standards: in the dental preparation, wearing the crown of1months and3months were recorded by the gingival crevicular fluid test teeth,gingival crevicular fluid in the amount of TNF–α and IL–1βlevels, anddetect the clinical index of plaque index (plaque index, PLI): referenceSilness and L e standard; gingival bleeding index (sulcus bieeding index,SBI): according to Mü hleman and Son standard; gingival sulcus depthprobing (gingival crevicular depth, GCD), namely the gingival crevicularbottom to the gingival margin distance, in millimetres. 3The filter strips prepared: Wharman Ⅲ type filter paper cut into filterpaper2×10mm, sterilization in aseptic table every four into a Eppendorfprior to disinfection and good (Ep) tube, electronic analytical balance with thenumber (precision) after weighing the stand-by.4Collection and preservation of the records of clinical index, gingivalcrevicular fluid samples: first recorded by the plaque index (PLI), and thenthe tooth collect GCF, using the scaler clean the dental plaque, cotton moisture,using filter paper strips in lingual near axial angle by drawing in gingivalcrevicular fluid after30seconds, put out, such as blood and salivacontamination is abandoned, returned to the original Ep tube sealedimmediately, weighing,-70℃frozen. Finally, the gingival crevicular gingivaprobing depth (GCD) and gingival index (SBI) measurement record.5Detection of TNF–α and IL–1βlevels: take the liquid before the EPpipe quality by using quality less liquid after EP tube, with the volume ofquality (approximately1mg/μL) converted into volume, can be obtained ingingival crevicular fluid volume number. The Ep tube removed thawing atroom temperature, adding250ml BSA–PBS buffer for each sample, to theshaker at room temperature oscillation1H, cryogenic centrifugal10min (4℃,10000r/min), the supernatant, measured by double antibody sandwich ELISAin gingival crevicular fluid TNF-α and IL-1β level.Results:1The comparison of indicators which repaired before the three PFM:The differences about the level of concentrations among the three groups before the repair of the tooth index, GCF, TNF-α and IL-1β don’t have any statistical significance(P>0.05).2A month after the repaired: Co-cr PFM crown group compared withbefore the level of GCD, SBI≥2, GCF volume and TNF–α and IL-1βconcentration levels significantly, the difference has statistical significance (P﹤0.05); Ti PFM crowns and zirconia all-ceramic crown group compared withbefore the level of GCD, SBI≥2, GCF volume and TNF-α and IL-1β，thechange of the concentration level is not obvious, and the difference has nostatistically significance (P>0.05). 3Three months after the repairation: Co-cr PFM crown group comparedwith the repair before the level of GCD, SBI≥2, GCF volume and TNF–αand IL–1β the concentration levels elevated obviously,the difference hasstatistical significance (P﹤0.05); Ti PFM crowns and zirconia all-ceramiccrown group compared with the repaired before the level of GCD, SBI≥2,GCF volume and TNF–α and IL-1β the change of concentration level is notobvious, and the difference has no statistically significant (P>0.05).4After repairation,the comparison between one month and three month:Co-cr PFM crown group after repaired the level of GCD, SBI≥2, GCFvolume and TNF–α and IL–1β, the concentration level increasesprogressively.The difference of the concentration level has statisticalsignificance (P﹤0.05); Ti PFM crowns and zirconia all-ceramic crown groupcompared to one month and three months after the restoration of thelevel of GCD, SBI≥2, GCF volume and TNF–α and IL–1β, the difference ofthe concentration level has nostatistically significant difference (P>0.05).5The comparison between one month and three month Co-cr PFMcrowns with Ti PFM crowns and zirconia all-ceramic crown group than in thelevel of GCD, SBI≥2, GCF volume, concentration of TNF–α and IL-1βlevel has a significant difference was statistically significant (P﹤0.05); TiPFM crowns with zirconia all-ceramic crown group after the restoration of thelevel of GCD, SBI≥2,GCF volume and TNF–α and IL-1β between twogroups, the difference of the concentration level has no statistically significant(P﹥0.05).6The frequence of PLI≥1before repaired, repaired after one month orthree months or the restoration of PFM crown had no obvious change, thedifference has no statistical significance (P﹥0.05).Conclusion:1The level of GCD, SBI≥2, GCF volume and levelofTNF–α and IL–1β of before repair, restoration one month and three monthsafter, co–cr PFM crown all showed a trend of increasing, Ti–PFM crown andzirconium dioxide all ceramic crown rises are not obvious. 2Compared with3groups of crowns, Co-Cr PFM has the most influence on periodontal health. Then,titanium porcelain crown and two zirconia all-ceramic crown have less influence than Co-Cr PFM, and both of them don’t have any significant effect on periodontal tissue.3Compared with3groups of crowns, Co-Cr PFM has the most influenceon periodontal health. Then,titanium porcelain crown and two zirconiaall-ceramic crown have less influence than Co-Cr PFM, and both of themdon’t have any significant effect on periodontal tissue. |
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