Effect Of Different PFM Materials On The Level Of TNF-α And IL-6 In Gingival Crevicular Fluid

Objective:To investigate the changes of gingival crevicular fluid(GCF)volume and the level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in GCF during different materials porcelain-fused-to-metal(PFM)crowns restoration.The relationship between the the level of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and the health of periodontal tissue was discussed and the effect of different inner crown materials on periodontal tissue was explored.Methods:The anterior teeth of 30 patients by random were conceived the restoration of Ni-Cr alloy PFM,gilt Ni-Cr alloy PFM,auro-galvano-crown PFM,and Au-Pt alloy PFM.Under the circumstances of strictly limiting crown axial contour,margin adaptation and periodontal conditions,the margin of crowns,a total of 40 teeth selected.They were divided into 4 groups,of which per group were 10 teeth.GCF was collected from labial and lingual of mesial site and distal site before the tooth preparation by 3MM filter paper.At the point of 1 month and 6 months after cementation,GCF collected at the same sites.At same time,clinical observation indexes were recorded.Before preparation,the volume of GCF and the level of IL-6 and TNF-αwere regarded as the baseline.The level of IL-6 and TNF-αwere detected by ELISA(enzyme-linked-immunosorbent assay)method,and analyzed the variation between the pre-restoration and post-restoration.Results:1.Compared to pre-restoration,the clinical observation indexes,the volume of GCF, the amount of TNF-αin GCF have marked difference in Ni-Cr alloy group after 1 month and 6 months.2.Compared to pre-restoration,the clinical observation indexes,the volume of GCF, the amount of TNF-αin GCF have no conspicuous difference in gilt Ni-Cr alloy group after 1 month;however,they have marked difference after 6 months.3.Compared to pre-restoration,the clinical observation indexes,the volume of GCF, the amount of TNF-αin GCF have no conspicuous difference in auro-galvano-crown and Au-Pt alloy group after 1 or 6 months.4.Compared to gilt Ni-Cr alloy group,the clinical observation indexes,the volume of GCF,the amount of TNF-αin GCF have marked difference in Ni-Cr alloy group after 1 month;however,they have no conspicuous difference after 6 months.5.Compared to auro-galvano-crown and Au-Pt alloy group,the clinical observation indexes,the volume of GCF,the amount of TNF-αin GCF have marked difference in Ni-Cr alloy group afterl or 6 months.6.Compared to auro-galvano-crown and Au-Pt alloy group,the clinical observation indexes,the volume of GCF,the amount of TNF-αin GCF have no conspicuous difference in gilt Ni-Cr alloy group after 1 month;however,they have marked difference after 6 months.7.Compared to Au-Pt alloy group,the clinical observation indexes,the volume of GCF,the amount of TNF-αin GCF have no conspicuous difference in auro-galvano-crown group after 1 or 6 months.8.Among the four kinds of crowns,there were no significant differences in IL-6 level at the 1 or 6 months.Conclusions:1.Different metal base alloys had different effects on the gingiva.2.Compared to auro-galvano-crown and Au-Pt alloy,Ni-Cr alloy and gilt Ni-Cr alloy were susceptible to inflammation of the periodontal tissue.Therefore,we should take noble metal PFM as possible as we can in the clinic.3.These 5 indexes,namely GI,GCD,GCF,IL-6 and TNF-α,can reflect the effect of different metal base alloy of PFM on the gingiva.Being used as evaluating indicators,they were definitely valuable for appraising the clinical effect of different PFM.