The Change Of Coagulation And Fibrinolysis System After Rat Exposured To Different Dose Of PM_2.5

Objective:With high incidence, cardiovascular disease is common in theworld. It is seriously harmful to human health. Cardiovascular disease areclosely related to eating habits,lifestyle, environment, genetic factor and manyother factors. Many studies found that exposure to particulate matter in the airpollution associated with many diseases, and it has the greatest impact on thecardiovascular system. Many studies have shown that exposure to particulatematter could increase the morbidity and mortality of cardiovascular disease,and ischemic cardiovascular events accounted for a large proportion. Exposureto particulate matter may greatly increase the risk of thromboembolism.Diabetes is one of the important independent risk factor of cardiovasculardisease. It can further aggravate the risk of cardiovascular disease afterexposuring to particular matter. However, the exact mechanism is not clear.This experimental purposes is to study the impact of particulate matter on thecoagulation and fibrinolysis system of normal rats and diabetic ratsintratracheal instillation of different concentrations of PM_2.5, detecting in ratblood PT, APTT, Fib, PAI-1levels.Methods:Total60rats were chosen, including30male Wistar healthyrats and30male Wistar diabetes rats. These rats were divided into3groupsrandomly: saline group, the low-dose group （7.5mg/kg） and the high-dosegroup （15mg/kg）, by intratracheal instillation of particulate matter, andanimals were sacrificed after24hours. The level of plasma PT, APTT and Fibwere detected by full-automatic coagulation analyzer, and the level of PAI-1was detected by ELISA method.All quantitative data which comply with the normal distribution wereexpressed as the means±SD （X±s）. For the statistical analysis of differencebetween the groups, analysis of Independent-Sample Test was used. For the statistical analysis of difference within the groups, analysis of SNK Test wasused. P<0.05had significant statistical significance. In this study, SPSS13.0statistical software was used to analyses statistics.Results:According to intratracheal instillation of PM with differentconcentration, the data of level of PT, APTT, Fib and PAI-1were analyzed byusing Excel2003and SPSS13.0statistical software.Compared with the saline group of healthy rats, PT decreased, Fib andPAI-1increased in groups of infusing particulate matter, P<0.05,the differencewas statistically significant.The change of above indicators were more obviousin high concentrations of particulate matter group.Compared with the saline group of diabetic rats,PT decreased, Fib andPAI-1increased in groups of infusing particulate matter, P<0.05,the differencewas statistically significant.The change of above indicators were more obviousin high concentrations of particulate matter group.The differences of APTT between different dose groups of healthy rats ordiabetic rats, P>0.05, the difference was not statistically significant.The variation differences of PT, Fib, PAI-1between healthy rats anddiabetic rats group infused of particulate matter in the same dose group, P<0.05, the difference was statistically significant.Conclusion:Our experimental results show that inhaled particulatematter can lead to a change of the rat coagulation and fibrinolysis system.With increasing the concentration of particulate matter, the impact on the ratcoagulation and fibrinolysis system is more severe; particulate matter mayhave an impact on the coagulation system mainly through the influence of theextrinsic coagulation pathway; the effects of particulate matter on coagulationand fibrinolysis system in diabetic rats are more pronounced.