| Background The treatment of infected bone defects remains an intractable problemand the ideal approach is to control infection and repair the bone defect at the sametime. A new calcium sulphate composite consisting of vancomycin and rhBMP-2wasdeveloped, and this study assessed its efficiency both in vitro and in a rabbit femurdefect model.Methods Firstly,the antibacterial properties and sustained-release effect of thecomposite material were detected in vitro. Secondly, chronic osteomyelitis model ofNew Zealand rabbit was established by methicillin-resistant Staphylococcusaureus.The compound scaffold is transplanted into bone defect of femur after chronicosteomyelitis is debrided, and whose ability of antiinfection and osteogenesis isassessed by the methods of radio-imageology, micro-CT and histopathology.Results The antibacterial ratio of rhBMP-2/VCM/nHA/CSH was more than99.8%.Moreover, the inhibition zone of18mm was formed in Staphylococcus aureusbacterium incubation dish with rhBMP-2/VCM/nHA/CSH disc for18hours ofincubation. Concentration of antibiotic sustained-release gradually reduced, theconcentration of antibiotics in the17th days are still very good.The results ofX-ray,Micro-CT and histopathology showed that there was no infection and lots ofnewly formed bone trabecula in transplanted site.Conclusion This composite may be a potential therapeutic agent for contaminated orinfected bone defects due to its concomitant osteoinductive and antibiotic properties. |
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