Effect Of Water-extraction From Massson Pine Pollen On Appetite And Lipid Metabolism Of Mice

Pine pollen, named Song Huang in Chinese traditional pharmacy, has functions in losing weight and tonifying recorded in ancient medical book. Recently, numerous studies about pine pollen on lipid metabolism showed that pollen can prevent or cure more than60kinds of diseases of human beings. However, the specific mechanism has not been scientifically understood.In our study, we used PWE from broken pollen of masson pine (Pinus massoniand) to investigate the preventing effect on diet-induced obesity of PWE on mice, which may provide basis mechanism in the development and application of the pine pollen in the field of weight loss. We mainly studied in the fild as follows:the final weight, weight net increase, weight gain rate, lipid levels in serum, lipids in mice feces, adipokines levels in serum, CPT-I concentration in liver and fat, CPT-I and FAS mRNA expression levels in liver, neuropeptide concentration in serum and neuropeptide mRNA expression levels in hypothalamus.In order to investigate the preventing effect of water-extract from masson pine pollen (PWE) on appetite and lipid metabolism of mice. In our study, sixty male KM mice (bodyweight was19±g) were divided randomly into six groups: normal control group (NC), high fat group (FC), positive control group (orlistat,0.05g/kg-d, PC), water-extract low-dose group (0.0625g/kg-d, PWEL), medium-dose group (0.125g/kg-d, PWEM) and high-dose group (0.25g/kg-d, PWEH). Mice were treated by intragastric administration daily for6weeks.The result shown as follows:the final weight, weight net increase and weight gain rate of three water-extract groups decreased obviously compared with FC group. Body fat content had slightly decreased but did not show significant difference. In serum, TC of PWEH group reduced remarkably (P<0.05, vs. FC group); TG of three water-extract groups had significantly declined (P<0.01, vs. FC group); HDLC of PWEM and PWEH groups had significantly increased (P<0.05, vs. FC group). TC and TG concentration of FC group in mice feces mostly higher than water-extract groups. Leptin level of PWEM and PWEH groups had significantly increased (P<0.01, vs. FC group). Adiponectin and resistin level of water-extract groups increased remarkably (P<0.01, vs. FC group). The activity of CPT-I of water-extract groups all had significantly increased (P<0.01, vs. FC group). Orexigenic peptides NPY and AgRP concentration in serum of water-extract groups had significantly declined (P<0.01, vs. FC group). Anorexigenic peptides POMC had no difference with each other, and CART has declined in different extent.In a word, feeding masson pine pollen water-extract could significantly improve lipid metabolism on diet-induced obese mice. PWE could not only promote the absorption and synthesis of fat but also promote the oxidation of fat. Critical was that the oxidation was stronger than the synthesis.