The Relationship Between Circulating MiRNA-142-5p And Acute Coronary Syndrome

Background:Acute coronary syndrome, is a major cause of cardiovascular disease to cause death and disability. The most common cause of coronary thrombosis is plaque rupture by plaque erosion. Traditionally,we determine the CAD severity according to the degree of the coronary stenosis.But we may find some patients who has the low-grade coronary stenosis to suffer from ACS.Just because the plaque is unstable.So the plaque that is stable or no seems to be more important for the ACS. Atherosclerosis, formerly considered a bland lipid storage disease, actually involves an ongoing inflammatory response. Recent advances in basic science have established a fundamental role for inflammation in mediating all stages of this disease from initiation through progression and, ultimately, the thrombotic complications of atherosclerosis. These new findings provide important links between risk factors and the mechanisms of atherogenesis. Clinical studies have shown that this emerging biology of inflammation in atherosclerosis applies directly to human patients. Elevation in markers of inflammation predicts outcomes of patients with acute coronary syndromes, independently of myocardial damage. In addition, low-grade chronic inflammation, as indicated by levels of the inflammatory marker C-reactive protein, prospectively defines risk of atherosclerotic complications, thus adding to prognostic information provided by traditional risk factors.miRNAs are present in various biological fluids including blood,and the levels of individual miRNAs and specific miRNAs signatures are linked to the diagnosis and prognosis for disease.We find that miRNA-142-5p is up-regulated in vulnerable plaque.So,it may be related to plaque’s stability.My test studies the relationship between miRNA-142-5p with acute coronary syndrome from a clinical point.and explore the relationship between it and inflammatory factors HsCRP and Fib.Aims:1.To study the diagnosis value of miRNA-142-5p to ACS.2.To study the relationship between miRNA-142-5p and CK-MB, cTnl.3.To study the relationship between miRNA-142-5p and inflammatory factors Hs-CRP and Fib.Methods:Blood samples for miRNA detection were collected from43ACS patients(who met inclusion criteria, including32AMI,11UAP),16stable CAD and16healthy volunteers from Qilu hospital. Total RNA in plasma were isolated by TRIzol LS. miRNA were quantified by using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assay. At the same time.test the cTnI,CK-MB,Hs-CRP,Fib in emergency laboratory of Qilu hospital. All results were analyzed by the software SPSS17.0.Results:1.miRNA-142-5p was detected in serum with qRT-PCR.is up-regulated in serum of ACS than stable CAD and healthy volunteers(P<0.05).There are no difference between stable CAD and healthy volunteers.2.There are no significant relationship between miRNA-142-5p and cTnl.miRNA-142-5p and CK-MB were positively correlated(r=0.389, P<0.05).3.miRNA-142-5p and inflammatory factors Hs-CRP and Fib were positively correlated (r=0.500.0.557, P=0.001).Conclusion:miRNA-142-5p has a certain value to diagnosis the ACS. and myocardial damage did not effect on miRNA-142-5p in plasma. The relationship between miRNA-142-5p and the biomaker of myocardial damage is weaker than that between miRNA-142-5p and inflammatory factors HsCRP and Fib, suggesting the gene may indicate the stability of plaque, but further research is needed.