Effect Of Apigenin On Testicular Redox Balance And Functions Of Mice

Objective To explore the effect of apigenin on testicular redox balance and functions of mice, in order to provide a reference basis for further study, and utilization of apigenin.Methods According to the body weight,200adult specific pathogen free male mice were divided into control group and three experimental groups(each group of50). Mice in high-dose group were given apigenin of1008mg/kgbw by intragastric administration, medium-dose group504mg/kgbw, low-dose group252mg/kgbw and control group were given normal saline (10ml/kg).10mice in every goup were killed in every7days. Mice’s serum E2and T were tested by radioimmunoassay,The quality and morphology of mice’s sperm were respectively tested by WLJY-9000and light microscope. Colorimetric method was used to test the activity of LDH, ACP, AKP, SDH, NOS, T-SOD, CAT and GSH-PX and the concentration of MDA, GSH and GSSG. The concentration of G-6-PD and NADPH were tested by enzyme linked immunosorbent assay.Results (1)There was no significant difference in mice’s weight among experimental groups and control group except in medium-dose group at14-day (P<0.05).The differences of organ coefficients of testis and epididymis were not statistically significant among experimental groups and control group at each observation point(P>0.05).(2)The sperm mean angular displacement of mice in medium-dose group was significantly lower than control group at14-day (P<0.05). The sperm velocity straight line and wobble in low-dose group were lower than control group at21-day (P<0.05). The sperm linearity and straight in high-dose group were significantly lower than control group at28-day (P<0.05).(3)The sperm abnormality rate in experimental groups were higher than control group at7-day (P<0.05),the medium and high-dose groups higher than control group(P<0.01) at28-day, the experimental groups higher than control group(P<0.01) at21-day and35-day.(4)The differences of concentration of T among experimental groups and control group were statistically significant at14-day (P<0.05), in low and medium-dose groups were lower than control group at21-day (P<0.05). The concentration of E2in medium-dose group was lower than control group at21-day (P<0.05).(5)The differences of activity of ACP between medium-dose group and control group was statistically significant at7-day (P<0.01). The activity of AKP in high-dose group was lower than control group at7-day (P<0.05). The concentration of G-6-PD in medium and high-dose group were higher than control group at14-day (P<0.05), and low-dose group higher than control group at35-day. The activity of LDH in experimental groups were higher than control group at21-day (P<0.05), the activity of CAT in low and medium-dose groups higher than control group at7-day (P<0.05) and T-AOC in low and medium-dose groups higher than control group at7-day (P<0.01).Conclusion Apigenin could affect the secretion of T in testis,cause a series of changes in sperm quality and morphology enhance the total antioxidant capacity at early experimental stage. However, the total antioxidant capacity could be reduced by large doses of apigenin with time increase.Testicular marker enzymes(ACP,AKP,G-6-PD) and antioxidant indexes (CAT,T-AOC) could be significantly changed in7～14d and be treated as sensitive index of early effect.