A Study Of The Manifestation Differences Of CDs In Infiltrating Cells Of Lesion Between Segmental Vitiligo And Non-segmental Vitiligo

BackgroundVitiligo is a common pigmentary disorder of the skin. World wide incidence of vitiligo is0.1%～2%.Vitiligo is classified into generalized and segmental type. This classification is of value because the course of vitiligo is different depending on its type.Segmental vitiligo, a subtype of vitiligo, is characterized by its early onset, rapid stabilization and unilateral distribution. The reported prevalence of segmental vitiligo ranges from3·5%to20·5%of all patients with vitiligo.In generalized vitiligo there is accumulating evidence for an autoimmune response against melanocyte self-antigens. In segmental vitiligo the underlying mechanism is still controversial.Based on the significant clinical differences between segmental and nonsegmental vitiligo, both types are in general considered to be separate entities. Associated autoimmune diseases in patients with segmental vitiligo and their family members are reported less frequently than in generalized vitiligo.ObjectiveTo explore the differences of immune response between segmental and non-segmental vitiligo Materials and Methods1.38cases of vitiligo lesion tissue were collected,which were all from our hospital outpatient deparment.Inclusion criteria:①age14-45years old;②clinical diagnosis in line with classification criteria of the pigment disease study group of the Dermatology Professional Committee of China Integrative Medicine Association③Within1year white macule expanded or new rash;④without hypertensive heart disease and other serious systemic disease;⑤without any treatments or within4weeks without oral immunosuppressants and in2weeks without opical medications;⑥voluntary participation. Normal controls selected from the plastic surgery deparment in the same period.2. The tissue was stained by HE.The expression of CD3, CD4, CD8, CD20, CD45RO, CD68, CD79a was detected by immunohistochemical staining. The images were saved by microscope camera for later analysis using Image-pro-plus6.0software. After the HE stainning,the morphology and the number of the melanophages were observed. The images were saved by microscope camera for later analysis using Image-pro-plus6.0software.3. Statistics analysis:All the statistical analyses were performed by the SPSS10.0software and all the data are expressed with X±S. The comparisons between groups were carried byANOVA. The inspection level is a=0.05.Results1. The manifestation of CD3, CD4, CD8, CD45RO and CD68of the infiltrating cells in non-segmental vitiligo was significantly higher than that of normal control group, the difference was statistically significant (P<0.01).(tablel)2. The manifestation of CD3, CD4, CD8, CD45RO and CD68of the infiltrating cells in segmental vitiligo was higher than that of normal control group, the difference was statistically significant (P<0.05).(tablel) 3. Compared with normal control group, the manifestation of CD20, CD79a of infiltrating cells are negative both in segmental and non-segmental vitiligo, there was no significantly statistical difference (P>0.01).(tablel and2)ConclusionsThe manifestation of CD3, CD4, CD8, CD45RO and CD68are positive both in segmental vitiligo and non-segmental vitiligo, so immune-mediated inflamination may play a role in the two kind of vitiligos.For non-segmental vitiligo, immune plays a main role; For segmental vitiligo, neuropeptides and cytokines are even more important.