| Objective:Current along with the development of the industry, causingenvironmental pollution is becoming more and more serious, in the productionof chemical materials and use, we found that the pollution of theenvironment’s influence on human health is becoming more and more obvious,and even the next generation of hazards to human health, also makes thehuman to attach great importance to this phenomenon, and bisphenol Abisphenol A particularly compelling. Bisphenol A (bisphenolA, BPA) is oneof the main raw material production of epoxy resin, polycarbonate, widelyapplied to the production of human life, such as: plastic products, babysupplies, dental implants, etc. Along with the use of the above items,bisphenol A (bpa) on the environment caused by pollution is increasinglyserious, had A great influence on human health. Male fertility tends to declinein recent years, foreign people Davis et al.[1]reported that the human spermdensity was down about50%from1940in1990, and semen volumedecreased by25%, and the incidence of male reproductive system dysplasiaincreased nearly1times, coverage analysis indicates that this phenomenon isrelated with the environmental estrogen exposure, which further shows thatenvironmental pollution becomes one of main factors to the decline in malefertility. Others reported that gave birth to a large number of sperm cellsapoptosis may be BPA caused; At the same time, still can cause testicularvolume increase is Fas and FasL protein expressed in the[2]. Now about theimpact of environmental estrogens on germ cell apoptosis study reported moreand more, but most of the research is to use the research methods of limitationand cell level and the whole environment hormone bisphenol A. We change Apoint of view, therefore, in vitro with low, medium and high doses of BPA virus induced in vitro cultured alone testicular sperm cells, bisphenol A (BPA)induced testicular germ cell apoptosis and Fas/FasL relations with C-mycgene. Impact on male fertility in recent years research has highly been globalscientists attention and research.This experiment using bisphenol A lavage infected male SD rats model tomonitor the germ cell apoptosis and Fas/FasL with C-myc gene expression intestis of rats, and to further explore the Fas/FasL and C-myc gene mediatedcorrelation between germ cell apoptosis pathway. For BPA fundamentalcauses of the germ cell apoptosis mechanism provides the theoretical basis,and for the prevention and control for chemical pollution caused by animaland human reproductive ability to provide new clues to expect further in orderto prevent and resolve environmental hormone bisphenol A (BPA) provide Abasis for human male fertility.Materials and methods：1experimental animals and materials: experimental animals to chooseclean level8weeks adult male SD (Sprague wrote-Dawley) rats, weight in180-200-g. Divide all the rats cage in the animal room for a week after theexperiment, the simulated natural lighting for12hours a day, are free to gatherthe food full price feed particles, drinking water, every canister regularly,canister to5times a week, and might be used in experiments after8weeks.2grouping and infected method: experimental animals will only40ratswere randomly divided into four groups: control group, low dose group,middle dose group and high dose group,10in each group. Each groupseparately in corn oil mixture concentration of different doses of BPA lavageinfected, low dose group was given2mg/kgBPA lavage, medium dose groupwas given20mg/kgBPA lavage, high dose group was given200mg/kgBPAlavage, control the daily irrigation amount of corn oil.3experimental specimen processing: after the infected rats weighing, andthen executed to weighing the epididymis testicular viscera coefficientcalculation, testicular neutral formaldehyde fixation, embedding, sectioninglines of HE staining and immunohistochemical staining, the contrast analysis of infected group and the control group, the application of SPSS software foranalysis.Results:1The rat behavior change:we found that the high dose group of ratsshowed:be hair messy,decreased activity,irritability,loss ofappetite, and appearthe symptom such as soft and;While the other group behavior did not seeobvious changes in rats.2The significance of rat body weight and testicles weight after weighingthe weight dose group and high dose of infected rats compared with controlgroup rats weight increased slowly, the difference was statistically significant(P <0.05), low dose group rats weight difference is not statistically significantcompared with control subjects (see Fig.1, Table1). Each canister to low dosegroup of rat testis weight slightly lower compared with the control group, butno statistical significance in the dose group and high dose group comparedwith control group difference was statistically significant (P <0.05(see Fig.2,Table2).3Rats testicles HE dyeing results of the analysis: to observe the rattesticular tissue structure of the control group found that the testiculardevelopment, fine tubular ordered more, SSCS, spermatocyte and mature at alllevels of progressive sperm cells arranged in rows, lumen contains a lot ofsperm to survive. Poor testicular development in medium and high dose group,arrange scattered fine tubular, mesenchymal cells is loose, and some finetubular cavity reproductive cells arranged disorderly. Slices of rats in thelower dosage treatment groups and control group no significant change.4Experimental control group, low dose group, middle dose group andhigh dose group of Fas/FasL and C myc gene and cryptorchidism germ cellapoptosis changes are shown in table. The results showed that low dose groupcompared with control groupTesticular germ cell apoptosis occurred by increase (P>0.05), Fas/FasLand C myc gene expression was also no significant increase (P>0.05);Medium and high dose group and low dose group compared with control group in apoptosis of testis germ cell increased significantly, Fas/FasL and Cmyc gene expression was also significantly increased (P <0.01).Control group and low dose group of Fas/FasL in rat testis and C myc-no obvious change compared to corresponding AI, no statistical significance(P>0.05); But low dose group, high dose group and control group withFas/FasL in rat testis and C-myc, compared to corresponding AI haveobvious changes, expression of apoptosis index significantly increased (P <0.05). Dose group and high dose group of Fas/FasL in rat testis and C-myccorresponding AI Fas/FasL in testis of rats and compared with C-myc,compared to corresponding AI also change significantly, the apoptosis indexsignificantly increased (P <0.05).Conclusion: Environmental hormone bisphenol A canister(through themouth) of male rats, on the reproductive system of male rats, certain toxic, andwith the intake of bpa concentration reaches A certain dosage can affect ratbody weight and testicles weight change, and germ cell apoptosis, np-sh (thegreater the dose the bigger the impact germ cell apoptosis, apoptosis indexincreases with dose. Bisphenol A canister to testicular germ cell apoptosis ofthe rats induced by increased; Fas/FasL and the increase in C-myc geneexpression may is one of the important reasons for the germ cell apoptosisoccurred; Bisphenol A germ cell apoptosis may be induced rats with C-mycphase coupling of Fas/FasL apoptosis pathway regulating. |
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