Experimental Study Of Effect On Biological Behavior Of Gastric Adenocancinoma Cells Line By FasL,p21～(WAF1/CIP1)and B7-1 Genes Mediated Via Recombinant Adenovirus

Experimental study of effect on biological behavior of gastricadenocancinoma cells line by FasL ~21WAF1/CIPi and B7-1genes mediated via recombinant adenovirusAbstractPart I:Apoptosis of human gastric carcinoma cell line SGC-7901 induced by FasL gene transductionObjective The current study was desigent to examine whether Fas-L could induce apoptosis in human gastric cancer xenografts in nude mice. Methods An adenoviral expression vector with full length cDNA of FasL gene insert was constructed(Ad-FasL) and transfected into SCIC- 7901 cells. The effect of exogenous FasL gene on the growth of SGC-7901 cells was examined in vitro and in vivo. Results Expression of FasL gene in SGC-7901 cells was confirmed by FACS and RT-PCR.The in vitro growth of the Ad-FasL transfected SGC-790 1 cells was significantly inhibited (inhibition rate: 84%) as compared to mock (Ad-LacZ) transfected SGC-7901 cells. Colony-forming activity in vitro of the Ad-FasL transfected SGC-7901 cells was completely inhibited. Ad-FasL transfected cells resulted in apoptosis-associated alterations: adder ands on agarose gel eletrophoresis and cell shrinkage, nuclear chromatin condensation, firnation of membrane blebs and apoptotic bodies were observed by electron microscopy. The growth of SGC-7901 xenografts in nude mice was retarded by intra-tumol injection of Ad-FasL. Conclusion FasL enhanced apoptosis in human gastric cancer xenografts in nude mice, suggesting FasL gene may be a potential anticancer agent for gastric cancer treatment.