Polymorphisms Of The Non-muscle Myosin Heavy Chain9Gene(myh9) In Chinese North Han Patients With Esrd

Current medical treatment can not yet effectively prevent kinds ofchronic kidney disease (CKD) from progressing to end-stage renal disease(ESRD). Genetic factors may play key roles in the pathogenesis of CKD andESRD according to the common forms of familial cluster. The non-musclemyosin IIA gene (MYH-9) polymorphisms are associated with focalsegmental glomerulosclerosis (FSGS), non-diabetic ESRD and DM-ESRD(Diabetes mellitus ESRD) in African Americans and in European Americans.However, it’s not known to what extent these polymorphisms are present inother ethnics. In this study, three MYH-9single nucleotide polymorphisms(SNPs) including rs4821480, rs4821481, rs2032487which are identified asassociation with kidney disease were examined. In addition, the association ofthe MYH-9SNPs with infections (hepatitis B and hepatitis C) in ESRDpatients with sustaining hemodialysis were further studied in Northern HanChinese.Objective:To investigate the association between the MYH-9genepolymorphisms and the ESRD in Northern Han Chinese population.Methods:Case control studies were carried out, and211ESRD patientsand118healthy controls were enrolled. The genomic DNA were extractedfrom whole peripheral blood leukocytes. Polymerase chainreaction-sequencing based typing (PCR-SBT) was used for detecting SNPsand the genotype frequency, the allele frequency and the relation of linkagedisequilibrium were calculated. Chi square was used to test the significance.Results:1By our investigation, the main proportion that caused ESRD chronicwere glomerulonephritis(46.6%,97/209), following were diabeticnephropathy (20.2%,42/209) and hypertensive nephropathy (11.5%,27/209). Also, the prevalence rate of hepatitis C infection was higher than that ofhepatitis B.2Compared with the control group, no previously reported SNPs(rs4821480, rs4821481and rs2032487) were associated with ESRD (all theparticipants were TT genotype). Among five newly discovered SNPs(MYH119，MYH308，MYH489，MYH600and MYH616) in Chinese Hanpopulation, MYH489frequented differently between the two groups (Fisher’sExact Test, P=0.013) and Gâ†'A mutation observed only in ESRD patientswith OR of1.68(95%CI,1.53-1.85, estimated by only the ESRD patients).3In case control studies exploring the association of hepatitis B orhepatitis C infections in ESRD patients of sustaining hemodialysis, SNPs ofMYH-9including rs4821480, rs4821481, rs2032487, MYH119, MYH308，MYH489，MYH600and MYH616were genotyped and no evidence suggestedthe relationships.4Logistic regression inlcuding the factors, such as age, gender, residence,education and SNPs of MYH-9, showed that age were significantlycontributed to the incidence of ESRD.Conclusion:1In Chinese Han population, the major causes of ESRD maybe chronicglomerulonephritis, diabetic nephropathy, diabetic nephropathy. Hepatitisinfections of ESRD patients with sustaining hemodialysis were the maincomplications.2Gâ†'A mutation of MYH-9gene at locus of489maybe one reasonattributed to the occurrence of ESRD.3Hepatitis infections of ESRD patients with sustaining hemodialysismaybe not associated with MYH-9gene polymorphisms.