| Objective：To detection the level of serum insulin-like growth factor1(IGF-1)and leukemia inhibitory factor (LIF) in children acute leukemia and toexplore its clinical significance，as the judgment condition,evaluation ofcurative effect, guide clinical treatment and evaluating prognosis providecertain theoretical basis.Manner:1Cases and groups：This research was randomly selected30cases newlydiagnosed and untreated with children acute leukemia to be hospitalized in theblood group of department of padiatrics in the second hospital of Hebeimedical university from August2011to August2012,those treated as theuntreated group of the experiment. Among them there were16children withacute lymphoblastic leukemia(ALL), and the other14children with acutemyeloid leukemia(AML). After a month from first chemotherapy, all the30cases had achieved complete remission，which was treated as remission onemonth group. Then after six months with treatment observation, they reachedcontinued complete remission, and treated them as remission six months group.Meanwhile, as the1:1matching principle, choosed the healthy children withsame factor like gender, age, body height and weight as control group. All thechildren in the research should have not any disease of heart, brain, kidney andendocrine. They also had not the sexual development.2Sample collection, handling and the data measurement: All of objectswere extracted2ml fasting venous blood in the morning of7:00to8:00. Allvenous blood were placed in the promoting coagulation tubes, and then putthem in the ordinary centrifugal machine, with a speed of2500r/min after10min, serum samples were collected. Divided the samples equally and keptthem in the fridge at-80℃. Measured the level of serum insulin-like growth factor1and leukemia inhibitory factor.3Statistics analysis: SPSS13.0was used, and the data was described asmean±SD. Analysis methods include analysis of variance. P value of less than0.05was considered as statistical significance.Consequence:1Serum IGF-1levels: IGF-1level was (36.01±12.41) ng/ml inuntreated group.The level was(61.64±12.84) ng/ml in remission one monthgroup.The level was (64.94±16.19) ng/ml in remission six month group.Thelevel was (75.91±12.95) ng/ml in normal control group.The level of IGF-1inuntreated group was the lowest. The level of IGF-1increased gradually withprolonged treatment time. The differences among four groups had statisticalsignificance (P＜0.05),and IGF-1level had not statistical significance betweenremission one month group，remission sixmonth group and normal control group (P＞0.05).2Serum LIF levels: LIF level was （46.83±8.91）ng/ml in untreatedgroup.The level was（78.98±7.28）ng/ml in remission one month group.Thelevel was（85.63±8.58）ng/ml in remission six months group.The level was(97.36±9.36) ng/ml in normal control group. The level of LIF in untreatedgroup was the lowest. The level of LIF increased gradually with prolongedtreatment time. The differences among four groups had statistical significance(P＜0.05),and LIF level had not statistical significance between remission onemonth group，remission six month group and normal control group (P＞0.05)Conclusion:1The levels of serum IGF-1and LIF in untreated group was distinctlylower than the control group, so they could become a kind of detection indexof acute leukemia.2After complete remission in childhood acute leukemia, the levels ofserum IGF-1and LIF gradually rose to normal. We suggested that dynamicdetection the levels of serum IGF-1and LIF in childhood acute leukemia in different stages of treatment were contributived to judge development ofdisease and assess treatment effect. |
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