The Comparison Of Haptoglobin Content In Multiple Sclerosis And Neuromyelitis Patient’s Serum And Cerebrospinal Fluid

Objective: Multiple sclerosis and neuromyelitis optica are bothautoimmune inflammatory demyelinating disease of central nervous system.Their etiology and pathogenesis are not fully understood yet.In the beginningNMO is considered to be a subtype of multiple sclerosis, but in recent years,especially with the discovery of the specific antibody----NMO-IgG, it isrecognized that NMO is a independent type of disease different from multiplesclerosis. There are many differences between the two diseases in thepathogenesis, clinical manifestations, diagnosis, treatment and prognosis.Therefore timely and correct diagnosis of both diseases and takeingappropriate therapeutic measures are particularly important. The diagnosis ofMS and NMO must base on the patient’s history, symptoms, signs, laboratorytests, MRI. The potential molecular markers to distinguish the two diseases aredifficult to be used in clinical,because of the economic benefits, technicaldifficulty, the lack of restrictions the sensitivity and specificity. So looking forpotential molecular markers to distinguish the two diseases, and exploringpossible new treatment direction are particularly important. The haptoglobin isan acidic glycoprotein and widely present in the serum and body fluids ofhumans and other animals.Its synthesis and degradation are mainly in liver.Haptoglobin’s main function is to combine free hemoglobin in serum to formHpHb complex.Then Hb will be transported to liver to be metabolism, thuswhich can avoid the loss of the hemoglobin and iron through kidney and thedamage to kidney. Hp is one of the acute phase response proteins, and it playsimportant roles in antioxidant activity, inhibition of prostaglandin synthesis,anti-infective, inhibit bacteria, promote angiogenesis and other importantimmune roles. Haptoglobin has genetic polymorphism phenomenon, in recentyears, the haptoglobin as a marker loci with the disease to investigate the etiology and pathogenesis of certain diseases has become a new importantissue. In some research HP raised in NMO which reflect the activities of theimmune system.It can be speculated that haptoglobin participate in theinflammatory response in neuromyelitis by lymphocytes and other cells of theimmune regulation. Some othe studies have shown that the increase rate ofhaptoglobin in multiple sclerosis patients and neuromyelitis patients aredifferent, which may provide a new reference index for the diagnosis ofmultiple sclerosis and neuromyelitis.The purpose of this experiment is to use Immage800specific proteinanalyzer applications and immune rate nephelometry measure to do apreliminary study about the comparison of haptoglobin content in multiplesclerosis and neuromyelitis patient’s serum and cerebrospinal fluid.Method:1Sample collection: Collect samples of serum and cerebrospinal fluidaccording to the diagnostic criteria for multiple sclerosis andneuromyelitis.And collect control samples at the same times.2Sample processing: Collect samples in biochemical tube, andcentrifuge the sample with3000r/min lasting3min within half an hour.Thencollect the supernatant and store it in EP tube at-80℃. Centrifuge reshcerebrospinal fluid with3000r/min lasting3min,and collect the supernatantand store it in EP tube at-80℃.3Parameters measure: Use Immage800specific protein analyzerapplications and immune rate nephelometry measure to measure thehaptoglobin content of the samples.4Statistical methods: Use SPSS13.0statistical analysis software toanalysis. The homogeneity of variance test use P>0.1as the criteria.Homogeneity of variance of the two sets of data use t test and unequalvariances of the two sets of data use rank sum test. Use P <0.05as theinspection level. P <0.05is considered to be statistically significant.Results:1The haptoglobin content datas measured by Immage800specific proteins analyzer applications and immune rate nephelometry measure are as follows:The haptoglobin content in serum of the multiple sclerosis group is1.32±0.14g/l.The haptoglobin content in serum of the neuromyelitis group are1.43±0.91g/l.The haptoglobin content in serum of the normal control group is0.69±0.62g/l.None group of the haptoglobin content in cerebrospinal fluid reached thelower detectiable limit of Immage800specific protein analyzer.2All the results use SPSS13.0to analyze.The ultimate results are as follows:2.1The age and gender of multiple sclerosis group, optic myelitis group, andthe control group in our study have no difference (P>0.05) after statisticaltests.2.2The comparison of serum haptoglobin content between MS group and thecontrol group was P>0.05,which means the difference is not significant,andthere is no statistical significance.2.3The comparison of serum haptoglobin content between MS group andNMO group was P <0.05,which means the difference is significant,andthere is statistical significance.2.4The comparison of serum haptoglobin content between NMO group andthe control group was P>0.05,which means the difference is notsignificant,and there is no statistical significance.Conclusion1Immage800specific protein analyzer have many excellent featuressuch as convenient, inexpensive, high stability, but it can not be applied tomeasure the haptoglobin content of the cerebrospinal fluid.2The serum haptoglobin measured by Immage800specific proteinanalyzer measured is not enough to be used in clinically distinguish diagnosisof multiple sclerosis and neuromyelitis yet.