Determination On Selenium Concentrations And Species In Selenium-Enriched Products And Studies On Selenium Antagonism Against Mercury Injury

Selenium is an essential trace element.Its function is not only connection with body level,but also closely related to its species.In this paper,the highly effective liquid chromatography-inductive coupled plasma mass spectrometry (HPLC-ICP-MS)was applied for selenium speciation analysis in Se-enriched Bifidobacterium and Yeast.The content of Se in them was determined by the inductively coupled plasma mass spectrometry(ICP-MS).The results showed that the total selenium content was 168.11μg/g in the Se-enriched Bifidobacterium while it was 1127.54μg/g in the Se-enriched yeast.The forms of selenium products in these samples were very complex and contained many unknown selenide.But the Se-Met was the main form existing in the Se-enriched yeast. Even its percentage was more than 80%of the total selenium in Se-enriched yeast.The residents in different mercury-exposure levels were chosen to test malonaldehyde(MDA)concentrations in their urine by thiobarbituric acid technique.The data showed that mercury poison had a positive correlation with high MDA in urine and that the injury of individuals in high mercury-exposure level was more serious than in low level.It made conclusion that the supplement selenium for people on high mercury-exposure condition was helpful to reduce the content of Hg in human urine and that Se played an important role against mercury injury.