| Fluorine (F) is one of the nonmetal trace elements which is indispensable to organisms. Having strong electronegative, fluorine widely exists in the environment by fluoride. Appropriate fluoride is beneficial to prevent caries, to maintain nerve transmission, and to support calcium and phosphate of organisms. And yet, excessive intake of fluoride produce potent toxicity to some systematical function of organisms. In the previous researches, importance was attached to the damage of skeletal tissue by fluorine. But with the progress of study and fluoride was extensively applied in industry, agriculture and our life, recent researches pay more attention to the nonskeletal toxicity of fluorine.Selenium (Se) is one of trace element and necessary to organisms. Selenium extensively exists in nature by compound. Appropriate selenium can increase urinary excretion offluorine, lessen the function of lipid peroxidation(LPO) of fluorine, and decrease the damage to skeletal and non-skeletal tissue by fluorine which include liver, kidney, thyroid gland, and so on. Howerver, there are not many reports about the antagonism of selenium on male reproductive toxicity by fluorine. To investigate male reproductive toxicity of fluorine and the effect of combined selenium and fluorine on male reproductive system, this study was carried out. It is valuable in the deep study and prevention of fluorosis by providing some experimental datum.Materials and methods1. One hundred twenty healthy male Wistar rats whose weight were between 55 gramme and 65 gramme were divided into six groups at random. The 1st group was the control group given non-ion water. The 2nd group was selenium group given non-ion water at a dosage of 2. Omg/L sodium-selenite(Na2Se03) solution. The 3th group was fluorine group given non-ion water at a dosage of 150mg/L sodium fluoride (NaF) solution. The 4th group was the group of fluorine with low-dose selenium given non-ion water at a dosage of 150mg/L NaF and 0. 5mg/L Na2Se03 solution. The 5th group was the group of fluorine with middling-dose selenium given non-ion water at a dosage of 150mg/L NaF and 2. Omg/L Na2Se03 solution. The 6th group was the group of fluorine with high-dose selenium given non-ion water at a dosage of 150mg/L NaF and 4. Omg/L Na2Se03 solution. There were 20 rats in every group, 5 of which were used inanalyzing chromosome aberration of primary spermatocytes.2. On the 71th day of the experiment, All index of each groups were measured: (1) weighing the body, the right testis, and the right epididymides; meanwhile, calculating their organic coefficient; (2) observing the sperm counts, mobility, and aberration; (3) surveying the levels of the left testicular lactate dehydrogenase (LDH) and the left epididymides ade-nosine triphosphatase(ATP) ; (4) measuring the levels of serum testosterone (T) and luteinizing hormone (LH); (5) observing pathological alterations of testis; (6) analyzing chromosome aberration of primary spermatocytes.3. One-way analysis of variance (ANOVA) and nonpar ametric statistics test were used in the statistical test. There was significant difference when a was 0. 05.Resultsl.No significant differences were observed about weight, the right epididymides and its organic coefficient, and the right testicular organic coefficient (p>0. 05). The right testicular weights (gramme) of each group was 1.80 + 0.22, 1.82 + 0.12, 1.74 + 0.19, 1.72 + 0.13, 1.83 + 0.10, and 1.61 + 0. 11. The weight of right testicular in the 6th group was less than the 3th groups' (/KO. 05).2. The count (107/ml) of sperm in each group was 192.73 + 32. 37, 200. 47+43. 09, 128. 00 + 11. 40, 142. 87 + 33. 50, 149. 43 + 34. 87, and 125. 33 + 14. 76. The sperm count of the 3th group was less than the 1st and 2nd groups' (p<0. 05). The sperm countof the 5th group was higher than the 3th group' s (p<0. 05).3. The rate of sperm mobility(%) in each group was 73.67 + 6.51, 72.67 + 4.47, 62.07 + 6.26, 66.07 + 6.22, 68.20 +4.81, and 64.936.06. The sperm mobility rate of t...
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