Study On Relationship Among Expression Of NRG-1 In The Peri-Hematomal Brain Region, As Well As Excess Syndrome Of Stroke And Prostration Syndrome In Intracerebral Hemorrhage Patients

Objectives: To observe the expression of NRG-1 in the peri-hematomal brain region of Intracerebral Hemorrhage (ICH) patients,study the role of NRG-1 in secondary cerebral injury, and investigate into the relationship among Excess Syndrome of Stroke and Prostration Syndrome, the expression of NRG-1,volume of blood tumor,scores of NIHSS and GCS. To provide the molecular biology standard for the microcosmic syndrome differentiation with TCM to quest for a new treatment.Materials and methods: 28 patients with ICH recruited and divided into three groups: YIN excess group, YANG excess group and Prostration group. All were accord with the diagnosing points of ICH and must be able to be diagnosed as one of the syndromes based on the differentiation standards of TCM and undergone surgical operation,among which 14 were male and 14 were female. YANG excess patients was 13(7 were male and 6were female),YIN excess patients was 8(both male and female were 4) and Prostration Patients was 7(3 were male and 4 were female).6 normal brain tissue were taken as normal control group. We collected the perihematomal brain tissue in the course of evacuation of hematoma in ICH group. The NIHSS and GCS were used to score, the DUOTIAN formula was used to calculate the hemorrhage volume,the NRG-1 expression was detected with Western blotting method.Results:1.The expression of NRG-1 in the peri-hematomal brain region of ICH patients significantly increased in comparison with the normal control groups (P<0.01).There were positive correlation among the expression of NRG-1 and scores of NIHSS (P<0.01).There were negative correlation among the expression of NRG-1 and scores of GCS (P<0.01).The expression of NRG-1 showed obvious positive correlation with the hematoma volume (P<0.01).2. The expression of NRG-l,the hematoma volume,the scores of NIHSS and GCS were significant different among YIN Excess Syndrome,YANG Excess Syndrome and the prostration Syndrome in ICH(P<0.05).Various types according to the expression of NRG-1, the hematoma volume and the scores of NIHSS order for: Prostration Syndrome>YIN Excess Syndrome>YANG Excess Syndrome,the GCS is contrary.Conclusion:1. The NRG-1 level maybe provide the molecular biology standard for the microcosmic syndrome differentiation with TCM. Combining the expression of NRG-1 in brain tissue with the volume of bleeding,GCS and NIHSS score maybe provide the molecular biology standard for the microcosmic syndrome differentiation with TCM.2. The expression rate of NRG-1 in the peri-hematomal brain region of ICH patients significantly increased in comparison with the normal control groups .A relevant analysis of the degree of brain impairment and NRG-1, there was a significantly positive inter-relationship between the degree of brain impairment and NRG-1.NRG-1 may have participated in the neuron protecting of ICH,the function may be through inhibiting of apoptosis and inflammatory response or stimulating an increase in the remaining nerve growth factor and anti-apoptotic factor around the hematoma.