| Sesamin, which belongs to lipid-soluble lignan compounds, is a safe and natural anti-oxidant,these compounds have powerful antioxidant activity. Sesamin is one special ingredients in sesame seeds, which makes sesame oils more stable than other vegetable oils such as soybean oil, rapeseed oils and sunflower oils ect. Sesamin have many functional effects, such as anti-oxidant activity, potential anti-aging, regulating lipid metabolism and lowering the level of cholesterol in serum, promoting alcohol metabolism and protecting liver, enhancing the immunity of the organism, significantly inhibiting the influenza virus, sendai virus and tubercle bacillus. In agriculture, sesamin commonly uses as a synergist for pyrethrin. Sesame is primary source of sesamin in nature. Using native sesame as samples, CO2 supercritical fluid extraction (SCF) of sesamin, analysis of sesamin and antioxidant activity of sesamin in high-tempreture was investigated in this article. All these provide basis for the development of sesamin products.1. The extraction of sesame oil from sesame seeds was investigated by supercritical CO2 fluid extraction (SCF-CO2). The influencing factors of supercritical fluid of CO2 extraction(SCF) on extraction rate of sesame oil, such as the pretreatment of raw material, extracting pressure,extraction temperature,CO2 flow rate,extraction time and separating pressure and temperature, were studied systematically. The optimized conditions were determined as follows: working pressure 35MPa, extraction temperature 40℃, extraction time 3 hours, CO2 flow rate 15 kg/h, grinding granularity 40 mesh, separation pressure 5MPa,separation temperature 70℃. Under these conditions, the extraction rate of sesame oil is 55.6%.2. High-performance liquid chromatography was used to determine the content of sesamin in sesame oils extracted by supercritical CO2 fluid extraction. The chromatographic conditions were as following: Stationary Phases was Chromatographic column of Diamonsil (C18 250 mm×4.6 mm, 5μm), mobile phase (methanol∶water) was 70:30, flow rate of 1.0 mL·min-1, the detective wavelength was set at 290nm. There was a good linear relationship between the concentration of sesamin and absorption area value in the range of 5μg mL-1～200μg mL-1 as follow: y =72741x+105063 ( R = 0.9999). The recovery of standard addition was 95.51% (RSD=1.29%, n=6). The developed method,which is simple, accurate and sensitive , can be used to determine the sesamin concentration.3. The influence of sesamin addition on the oxidation stability of lard and soybean oil under high temperature treatment was investigated. The sesamin shows its excellent oxidative stability in high-temperature conditions. The peroxide value of contrast lard samples is 15.7 times as great as that of the lard samples added 0.10% of sesamin. The peroxide value of contrast lard samples is 4.1 times as great as that of the soybean oil samples added 0.10% of sesamin, which can prolong the frying time of lipid. The anti-oxidative effects of sesamin on the lard and soybean oils are better than those of tea ployphenol at the same concentration. There was more significantly synergistic effect on thermal antioxidation of sesamin for phosphoric acid than lactic acid and EDTA. |
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