Chitosan Quito Hole Research Of Membrane Material

The rapid development of biotechnology requires a higher level of protein separation.The key problem is to keep the biological activity of the protein.The traditional chromatographic column has not only the advantage of large column volume and efficient separation but also the disadvantage of low flow speed,high pressure,difficulty to enlarge.The traditional membrane separation can do fast separation in low separation and has the advantage of no phase transition,low energy consumption,easyenlargement,continuous work,wide range of material sources and low price.But with the screening mechanism,it cannot deal with mixtures similar in size effectively.Inchapter two, We successfullysynthesis chitosan-N-arginine, and characterize the product with many methods such as Solid13C NMR, FT-IR,XRD, then we prepared macroporous chitosan-N-arginine blend membrane as the matrix to set up a membrane chromatography for protein adsorption and separation.In chapter three, wepreparedmacroporous CS/CMC blend membrane as the matrix to set up a membrane chromatography for protein adsorption and separation. We selected lysozyme as model protein and investigated its dynamic adsorption property on the CS/CMC membrane chromatography extensively by the flow rate and the initial concentration of feed solution, as well as the layers of membrane in membrane stack. The results showed that the low flow rate and high initial lysozyme concentration was favorable to achieve a high dynamic adsorption capacity, and the multiple membranes in the membrane stack will increase the adoption ability also.In the last chapter four,wesummary and prospect of our research work.Our research worksmainlyfocus on the design of the membrane chromatography. We successfully designed and finished2chitosan-based membranes, these are chitosan-N-arginine and CS/CMC blend membrane chromatography.