Porous Array Membrane Structure Of Silver And Gold Nanorods Surface Enhanced Fluorescence Characteristics Research

Excited under the incident light field, the metal substrate surface which have nano-structure can produce surface plasmons, which will changes the electromagnetic field distribution near by the metal substrate, modulation fluorescence spectra properties of the fluorescent species which located in near the surface of substrate, so that get a higher fluorescent radiation efficiency compare with fluorescent species at free state, we put this phenomenon is called surface-enhanced fluorescence effect.More and more researchers begin pay attention to the study of Surface Enhanced Fluorescence effect,with the increasing demands of the engineering actual environment on the spectral detection sensitivity requirement. For this, people have a research on the effects of surface-enhanced fluorescence from nano-structured morphology, size, self-assembled structures, and made a lot of achievements in the theoretical and experimental. At present, Surface-enhanced fluorescence spectroscopy has broad application in the field of biomedical and chemical detection, which was owing to it’s high sensitivity has become one of the hot researches in the field of surface-enhanced spectroscopy.Researchers pay close attention to one of the focuses of the property and morphology of the substrate for the enhancement effect during the investigation into mechanism and application of surface enhanced fluorescence.Recently, with the development of nanotechnology, varying morphology of substrate for enhanced fluorescence such as nanowires, nanorods, nanoparticles have been prepared. There are two parts included in the thesis:in the first one the porous anodic aluminum oxide templates were maked through second anodic oxidation method and The Ag nanohole arrays structure was fabricated with deposited on the porous anodic aluminum oxide template by magnetron sputtering, and discussing the fluorescence enhancement of Rh6G fluorophore molecules from the Ag nanohole arrays as substrate. The second part deals with the fluorescence enhancement effect of PATP modified Gold Nanorods to AO molecules.The first partThe fluorescence enhancement effect of silver nanohole arrays The silver nanohole arrays were fabricated by deposing metal silver onto porous anodic aluminum oxide templates through magnetron sputtering method.The fluorescence enhancement of Rh6G molecule from the prepared silver nanohole substrates was investigated. The results showed that the silver nanohole arrays have better fluorescence enhancement effect compared with conventional silver surface. The enhancement factor presented a size and period dependence of the hole array, which was owing to the surface plasmon excitation and the coupling of enhanced local EM filedThe second partThe fluorescence enhancement effect of Gold nanorods to acridine orange molecules.To alter the distance between fluorophore molecule and metal surface on the fluorescence enhancement effect by changing the concentration of4-Aminothiophenol(PATP). The result shows that, with the increase the concentration of PATP molecules, the fluorescence enhancement factor first goes up to a maximum, then followed by a decrease. We discussed the experimental phenomena with energy transfer and local field enhancement theory. Have been reported that there have the best fluorescence enhancement factor with change the distance between the fluorescent molecules and the gold nanorods by changing the concentration of4-Aminothiophenol...