For Magnetic Nano Microsphere Used For Induction Heat Slow-release Chemotherapy Tumor

Objective:The purpose of this project is to study the sustained-release nano-magnetic composite microspheres carrying doxorubicin hydrochloride for the tumor magnetic induction mitigation thermo-chemotherapy. The advantages of the tumor magnetic induction hyperthermia and tumor drug delivery therapeutic will be integrated.To explore the inhibition of proliferation of human soft tissue sarcoma (HT-1080) and human breast cancer (MCF-7). In addition, we explore the anti-tumor effect and the feasibility with tumor-bearing mice models. And then comprehensive experimental and theoretical basis will be provided for clinical cancer treatment The purpose of this project is to study the sustained-release nano-magnetic.Methods:1、Preparation and characterization of drug-loaded magnetic nanocomposite microspheresPreparation of drug-loaded magnetic nanocomposite microspheres by chemical coprecipitation which can be wanned in the alternating magnetic field with oleic acid modified. Double emulsion prepared PLGA matrix containing anti-cancer chemotherapy drug doxorubicin hydrochloride and FesO4magnetic nano particles thermostat composite microspheres.The appearance of drug-loaded magnetic nanocomposite microspheres morphology, the drug internal distribution, heating performance in the external alternating magnetic field, in vitro drug release properties were characterized by the modem instrumental to determine the basic physical and chemical properties for the composite microspheres nature and conditions of the subsequent biological experiments.2、Drug-loaded magnetic nanocomposite microspheres in vitro of biocompatibility evaluationExtraction method was used to pretreat the material. Indirect contact method (Material extracts) was used to culture the mouse fibroblasts Department of L-929. The cell growth was observed at different time points status (including cell morphology, proliferation, etc.), and detected the cell proliferation by method of CCK-8, and ultimately evaluate the biocompatibility of drug-loaded magnetic nanocomposite microspheres.3、Drug-loaded magnetic nanocomposite microspheres in vitro of cancer therapyHuman fibrosarcoma HT-1080cell lines and human breast cancer MCF-7cell line were incubated with the drug-loaded magnetic nanocomposite microspheres, phagocytosis assay, using laser scanning confocal microscopy phagocytosis situation; comprehensive thermo-chemotherapy and compared the efficacy of the chemotherapy drug doxorubicin hydrochloride experiments. Detect the relative growth rate by CCK-8. According to the Veleriote method (Webber coefficient) calculate the final comprehensive evaluation contained drugs magnetic nanocomposite microspheres.4*Drug-loaded magnetic nanocomposite microspheres in vivo of cancer therapyThe cell suspension was injected subcutaneously human breast cancer cell line MCF-7cell lines method, on the BALB/c-nu mice model. Modeled tumor-bearing mice were randomly grouped. Inject the materials in the tumors. And treat the groups of tumor-bearing mice. The final tumor volume, tumor-bearing mice survival and vital organs biopsy was the evaluation index to comprehensive analysis of drug-loaded magnetic nanocomposite microspheres in vivo anti-tumor properties.Results:1、Preparation and characterization of drug-loaded magnetic nanocomposite microspheresDrug-loaded magnetic nanocomposite microspheres are spherical, smooth surface, having a hollow structure, and an average particle diameter of2.753μm. The drugs in the ball are distributed uniformly. When it be exposed to the external alternating magnetic field, the material of different concentrations showed good heating performance. Temperature-controlled drug release can be achieved.2、Drug-loaded magnetic nanocomposite microspheres in vitro of biocompatibility evaluationEmpty loaded composite microspheres with good biocompatibility, no significant cytotoxicity. Loading magnetic nanoparticles (MNPs) microspheres (except the the100mg/mL concentration) has good biocompatibility, no significant adverse effect on cell morphology and proliferation.3、Drug-loaded magnetic nanocomposite microspheres in vitro of cancer therapyHuman fibrosarcoma HT-1080cell lines and human breast cancer MCF-7cell lines showed some phagocytosis of drug-loaded magnetic nanocomposite microspheres; material thermo-chemotherapy toxic effects on the two cell lines was significantly better than the control group, There is a thermo-chemotherapy synergistic Sensitizing effect such increase in the role.4、Drug-loaded magnetic nanocomposite microspheres in vivo of cancer therapyDrug-loaded magnetic nano-composite microspheres mediated tumor treatment compared with control group with significant inhibition of tumor growth and treatment and prolong the survival time of tumor-bearing nude mice.Conclusion:Drug-loaded magnetic nanocomposite microspheres loading doxorubicin hydrochloride are biocompatibility. It can be used as magnetic induction chemotherapy media for soft tissue sarcomas (fibrosarcoma) sustained-release thermal and of breast cancer. It can simultaneously achieve a temperature-controlled drug release, biodegradable drug delivery, local thermo-chemotherapy therapeutic effect. Further, it can provide new ideas, new ideas and new value for comprehensive cancer treatment research and late-stage clinical applications.