The Hulunbuir Sandy Different Vegetation Restoration Mode Of Soil Microbial Diversity

Soil microbial, which is the main index to evaluate soil quality, plays a significant role in ecosystem, and soil microbial diversity is the important one of global diversity. Exploring the effect of different vegetation patterns on soil microbial diversity can provide scientific bases and technical support for systemic and impersonal assessment of the best vegetation restoration patterns and the vegetation restoration and reconstruction of Hulunbeier sandy land.In this paper, five vegetation restoration patterns were studied in sandy land in Hulunbeier, China. The changes of soil physical and chemical properties and soil enzyme activity were measured in the above five vegetation restoration patterns. Based on Biolog-Eco technology, a case study was carried out to investigate the effects of five different vegetation restoration patterns on soil microbial functional diversity. Using PCR-DGGE technology, analyzed the genetic diversity of soil bacterial communities. The results showed that:(1)With the five vegetation restoration patterns, the soil organic carbon content, the soil total nitrogen content, the soil total phosphorus content and the soil available phosphorus were significantly increased. The vegetation restoration improved the soil nutrient content significantly;(2)Compared to the control sandy land, the soil urease, alkaline phosphatase and catalase enzyme activities increased obviously, and the effects of different vegetation type on soil enzyme were significantly different. Besides, compared to0-10cm, soil enzyme activities in10-20cm decreased significantly. Soil urease, alkaline phosphatase and catalase enzyme activities showed stronger positive correlations with the soil nutrient content especially the soil organic carbon content, the soil total nitrogen content and the soil total phosphorus content.(3)The carbon source utilization ability of soil microbial community enhanced significantly after vegetation restoration. The vegetation restoration enhanced the metabolic activity and functional diversity of microbial community in sandy soil.(4)The vegetation restoration increased the genetic diversity of soil bacterial community obviously, and the structure of soil bacterial community were changed. The results suggested that the bacterial community in Hulunbeier sandy land mainly consisted of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Acidobacteria. The dominant groups are Proteobacteria and Bacteroidetes.(5) Comprehensive analysis of soil nutrients、 soil enzyme activities and soil microbial diversity in different vegetation restoration patterns, the ideal vegetation planting pattern in Hulunbeier sandy land is mixed planting of Hedysarum fruticosum、 Caragana korshinskii、 Agropyron cristatum and Elymus nutans is better than mixed planting of Caragana korshinskii and Agropyron cristatum, better than mono-cultivar planting of Hedysarum fruticosum、 Caragana korshinskii and Agropyron cristatum.